Elevation in surface enhanced fluorescence performance mediated by glutathione protonation in acidic environments.

IF 3.3 2区 物理与天体物理 Q2 OPTICS
Optics express Pub Date : 2025-07-28 DOI:10.1364/OE.567526
Shi Wang, Wenbo Wu, Yue Li, Chenxi Gu, Xiaolong Song, Yafang Pan, Zhen Sun, Yunfan Yang, Tifeng Jiao, Mingli Wang
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引用次数: 0

Abstract

Surface enhanced fluorescence (SEF) plays a key role in sensing and cell imaging. However, in order to prevent cytotoxicity, the concentration of noble metal nanoparticles (Ag) needs to be lowered, which leads to poor SEF performance. Herein, different Ag@C (core-Ag, shell-carbon) nanoparticles were synthesized, and Ag@C nanoparticles prepared under 4 h heating (AC4.0) had the largest SEF enhancement factor (EFsef). Dilution of AC4.0 markedly reduces EFsef. Subsequently, Ag@C-GSH nanoparticles (ACG) were prepared by modifying glutathione (GSH) on the surface of diluted AC4.0. EFsef of ACG was improved at pH 4, and similar result for diluted AC4.0 at pH 8. This recovery of EFsef at low nanoparticle concentrations was attributed to a mechanism of electric field strength enhancement: acid-induced protonation of glutathione mediates aggregation of ACG via hydrogen bonding, which amplifies electric field strength at "hot spots".

在酸性环境中,由谷胱甘肽质子化介导的表面升高增强了荧光性能。
表面增强荧光(SEF)在传感和细胞成像中起着关键作用。然而,为了防止细胞毒性,需要降低贵金属纳米颗粒(Ag)的浓度,这导致SEF性能较差。本文合成了不同的Ag@C(核-银,壳-碳)纳米颗粒,其中加热4 h (AC4.0)制备的Ag@C纳米颗粒具有最大的SEF增强因子(EFsef)。稀释AC4.0可显著降低EFsef。随后,通过在稀释的AC4.0表面修饰谷胱甘肽(GSH)制备Ag@C-GSH纳米颗粒(ACG)。在pH为4时,ACG的EFsef得到了改善,在pH为8时,稀释的AC4.0也得到了类似的结果。低纳米颗粒浓度下EFsef的恢复归因于电场强度增强的机制:酸诱导的谷胱甘肽质子化通过氢键介导ACG聚集,从而放大了“热点”处的电场强度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Optics express
Optics express 物理-光学
CiteScore
6.60
自引率
15.80%
发文量
5182
审稿时长
2.1 months
期刊介绍: Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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