Soil microbial legacy mediated by buckwheat flavonoids enhances cabbage resistance to clubroot disease.

IF 12.7 1区生物学 Q1 MICROBIOLOGY

Microbiome Pub Date : 2025-07-29 DOI:10.1186/s40168-025-02166-y

Jiabing Wu, Shilin Hu, Jing Chen, Lili Zhou, Shengdie Yang, Na Zhou, Lei Wu, Guoqing Niu, Yong Zhang, Xuesong Ren, Qinfei Li, Jun Yuan, Hongyuan Song, Jun Si

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引用次数: 0

Abstract

Background: The legacy of plant growth significantly impacts the health of subsequent plants, yet the mechanisms by which soil legacies in crop rotation systems influence disease resistance through rhizosphere plant-microbiome interactions remain unclear. Using a buckwheat-cabbage rotation model, we investigated how microbial soil legacies shape cabbage growth and clubroot disease resistance.

Results: Three-year field trials revealed that buckwheat rotation sustainably reduced clubroot severity by 67%-97%, regardless of pathogen load. Soil sterilization eliminated this suppression, implicating a microbial basis. Using 16S rRNA sequencing, we identified buckwheat-enriched bacterial taxa (Microbacterium, Stenotrophomonas, Ralstonia) that colonized subsequent cabbage roots. Metabolomic profiling pinpointed buckwheat root-secreted flavonoids - 6,7,4'-trihydroxyisoflavone and 7,3',4'-trihydroxyflavone - as key drivers of microbial community restructuring. These flavonoids synergistically enhanced the efficacy of a synthetic microbial community (SynCom1, containing Microbacterium keratanolyticum, Stenotrophomonas maltophilia, and Ralstonia pickettii), boosting disease suppression by 34% in greenhouse trials. Co-application of flavonoids and SynCom1 improved bacterial colonization in root niches. Although SynCom1 partially activated jasmonic acid (JA)-associated defenses, its effectiveness depended primarily on flavonoid-driven microbial recruitment rather than direct immune induction.

Conclusions: Buckwheat rotation induces flavonoid-mediated soil microbiomes that prime JA-dependent immunity in subsequent cabbage crops, thereby decoupling disease severity from pathogen load. This study elucidates how specialized metabolites orchestrate cross-crop microbial legacies for sustainable disease control, providing a blueprint for designing rotation systems through precision microbiome engineering. Video Abstract.

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荞麦类黄酮介导的土壤微生物遗产增强了白菜对根瘤病的抗性。

背景：植物生长的遗产显著影响后续植物的健康，但作物轮作系统中土壤遗产通过根际植物-微生物组相互作用影响抗病性的机制尚不清楚。利用荞麦-白菜轮作模型，我们研究了土壤微生物遗传如何影响白菜的生长和甘蓝的抗病能力。结果：为期三年的田间试验表明，无论病原菌负荷如何，荞麦轮作都能持续降低67 -97%的根茎病严重程度。土壤灭菌消除了这种抑制，暗示微生物基础。通过16S rRNA测序，我们确定了富含荞麦的细菌分类群（Microbacterium, Stenotrophomonas, Ralstonia），这些细菌在随后的卷心菜根中定植。代谢组学分析指出，荞麦根分泌的类黄酮- 6,7,4'-三羟基异黄酮和7,3',4'-三羟基黄酮-是微生物群落重组的关键驱动因素。这些黄酮类化合物协同增强了合成微生物群落（SynCom1，包含角化微杆菌、嗜麦芽窄养单胞菌和皮氏Ralstonia pickettii）的功效，在温室试验中将疾病抑制率提高了34%。黄酮类化合物和SynCom1共同施用可改善根生态位细菌定植。虽然SynCom1部分激活茉莉酸（JA）相关防御，但其有效性主要依赖于类黄酮驱动的微生物招募，而不是直接的免疫诱导。结论：荞麦轮作诱导黄酮类介导的土壤微生物组，在随后的卷心菜作物中启动ja依赖免疫，从而将疾病严重程度与病原体负荷脱钩。这项研究阐明了专门的代谢物如何协调作物间的微生物遗传以实现可持续的疾病控制，为通过精确的微生物组工程设计轮作系统提供了蓝图。视频摘要。

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来源期刊

Microbiome MICROBIOLOGY-

CiteScore

21.90

自引率

2.60%

发文量

198

审稿时长

4 weeks

期刊介绍： Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.