Nisa Fauziah, Yunia Sribudiani, Lia Faridah, Fedri Ruluwedrata Rinawan, Naufal Fakhri Nugraha, Muhammad Farhan Maulana, Dwi Agustian
{"title":"Molecular virology profiles of dengue cases in Bandung, Indonesia.","authors":"Nisa Fauziah, Yunia Sribudiani, Lia Faridah, Fedri Ruluwedrata Rinawan, Naufal Fakhri Nugraha, Muhammad Farhan Maulana, Dwi Agustian","doi":"10.4103/jvbd.jvbd_23_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Background objectives: </strong>Bandung City, West Java, Indonesia, is one of the hyperendemic areas for dengue virus (DENV) infections. However, data on virus distribution in this area is currently limited. This study aims to assess the distribution of serotypes and genotypes of DENV isolated from dengue patients in primary healthcare facilities (PHC) in 2018.</p><p><strong>Methods: </strong>A cross-sectional study was conducted in three PHCs in Bandung City from May to October 2018. Suspected dengue patients with a fever lasting than five days were enrolled. Demographic and clinical data were collected upon enrollment. Dengue NS1 rapid diagnostic test (RDT) were followed by RT-PCR and nested PCR performed on patients' serum samples. A phylogenetic tree was constructed based on E-gene sequence data.</p><p><strong>Results: </strong>A total of 236 suspected dengue patients were enrolled in this study, with 51 confirmed dengue infections based on the NS1 rapid test. Among these 51 cases, 23 (45%) were positive by RT-PCR (2 DENV-1, 6 DENV-2, 10 DENV-3, 3 DENV-4, and 2 with mixed DENV serotypes). The predominance DENV-3 in 2018 was consistent with the reports from 2008, indicating the same predominant serotype circulated in the area. Phylogenetic analysis revealed that DENV-1 genotype 1 and DENV-3 genotype 1 were circulating in Bandung.</p><p><strong>Interpretation conclusion: </strong>All four DENV serotypes were identified in samples collected from PHC Neglasari, Padasuka, and Sukajadi, confirming that Bandung is a hyperendemic areas. Strengthening vector control measures is crucial to prevent further transmission of dengue infection.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":" ","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Vector Borne Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/jvbd.jvbd_23_24","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background objectives: Bandung City, West Java, Indonesia, is one of the hyperendemic areas for dengue virus (DENV) infections. However, data on virus distribution in this area is currently limited. This study aims to assess the distribution of serotypes and genotypes of DENV isolated from dengue patients in primary healthcare facilities (PHC) in 2018.
Methods: A cross-sectional study was conducted in three PHCs in Bandung City from May to October 2018. Suspected dengue patients with a fever lasting than five days were enrolled. Demographic and clinical data were collected upon enrollment. Dengue NS1 rapid diagnostic test (RDT) were followed by RT-PCR and nested PCR performed on patients' serum samples. A phylogenetic tree was constructed based on E-gene sequence data.
Results: A total of 236 suspected dengue patients were enrolled in this study, with 51 confirmed dengue infections based on the NS1 rapid test. Among these 51 cases, 23 (45%) were positive by RT-PCR (2 DENV-1, 6 DENV-2, 10 DENV-3, 3 DENV-4, and 2 with mixed DENV serotypes). The predominance DENV-3 in 2018 was consistent with the reports from 2008, indicating the same predominant serotype circulated in the area. Phylogenetic analysis revealed that DENV-1 genotype 1 and DENV-3 genotype 1 were circulating in Bandung.
Interpretation conclusion: All four DENV serotypes were identified in samples collected from PHC Neglasari, Padasuka, and Sukajadi, confirming that Bandung is a hyperendemic areas. Strengthening vector control measures is crucial to prevent further transmission of dengue infection.
期刊介绍：
National Institute of Malaria Research on behalf of Indian Council of Medical Research (ICMR) publishes the Journal of Vector Borne Diseases. This Journal was earlier published as the Indian Journal of Malariology, a peer reviewed and open access biomedical journal in the field of vector borne diseases. The Journal publishes review articles, original research articles, short research communications, case reports of prime importance, letters to the editor in the field of vector borne diseases and their control.
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