Heat stress responses mediated by N6-methyladenine DNA methylation in maize.

IF 6.9 1区 生物学 Q1 CELL BIOLOGY
Weijun Guo, Liang Le, Daolei Zhang, Ziwei Wei, Yifan Wang, Yue Wu, Hada Wuriyanghan, Xiaofeng Gu, Li Pu
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引用次数: 0

Abstract

N6-methyladenine (6mA) plays an important role in eukaryotic development and stress responses, yet its function under heat stress (HS) in crops remains unclear. Here, we show that 6mA dynamics in two maize inbred lines, B73 and Mo17, correlate with their responses to HS. Genome-wide 6mA profiling reveals enrichment in promoters, intergenic regions, and transposable elements (TEs), with inverse correlation with gene/TE expression. Upon HS, heat-tolerant plants show elevated 6mA levels, with differential 6mA patterning on key HS-related genes underlying thermotolerance variation between B73 and Mo17. We identified ZmALKBH1 as a 6mA demethylase, and its mutation enhances HS tolerance. A deep learning model based on 6mA methylomes of B73 and Mo17 accurately predicts and experimentally validates 6mA distribution and HS response in additional W22 and B104 lines. These findings uncover the role of 6mA in transcriptional regulation of crop stress adaptation and offer potential targets for improving thermotolerance in maize.

玉米n6 -甲基腺嘌呤DNA甲基化介导的热胁迫反应
n6 -甲基腺嘌呤(n6 - methylladenine, 6mA)在真核生物的发育和逆境响应中起着重要作用,但其在作物热胁迫下的功能尚不清楚。本研究表明,两个玉米自交系B73和Mo17的6mA动态与其对HS的响应相关。全基因组6mA谱显示启动子、基因间区和转座元件(TE)富集,与基因/TE表达呈负相关。在高温胁迫下,耐热植物的6mA水平升高,B73和Mo17在高温胁迫相关关键基因上的6mA模式存在差异。我们发现ZmALKBH1是一种6mA去甲基化酶,它的突变增强了HS耐受性。基于B73和Mo17的6mA甲基组的深度学习模型准确预测并实验验证了额外W22和B104的6mA分布和HS响应。这些发现揭示了6mA在作物逆境适应转录调控中的作用,并为提高玉米耐热性提供了潜在的靶点。
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来源期刊
Cell reports
Cell reports CELL BIOLOGY-
CiteScore
13.80
自引率
1.10%
发文量
1305
审稿时长
77 days
期刊介绍: Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.
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