Complete genome analysis of a novel phenui-like virus in Morus alba

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-07-29 DOI:10.1007/s00705-025-06378-3

Hanze Zheng, Yangwei Gao, Xiaodi Hu, Zhongtian Xu, Jian-Ping Chen, Yiyuan Li

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引用次数: 0

Abstract

Mulberries (genus Morus) are important to sericulture. Due to interspecific hybridization and the existence of various cultivars, the virome of many Morus species is still unknown. In this study, we identified a novel negative-sense phenui-like virus in Morus alba and determined its genome sequence. The virus possesses three linear single-stranded negative-sense RNA segments: RNA1 (7,329 nt), RNA2 (1,276 nt), and RNA3 (1,222 nt). Each of the three segments contains an open reading frame (ORF), encoding the RNA-dependent RNA polymerase (RdRP), the movement protein (MP), and the nucleocapsid protein (N), respectively. Phylogenetic analysis placed this virus within a clade including members of the genus Rubodvirus (family Phenuiviridae, order Hareavirales, class Bunyaviricetes) with raspberry rubodvirus 1, grapevine Muscat rose virus, and apple rubbery wood virus 2 being the closest relatives. Small interfering RNA (siRNA) sequencing data suggested that the virus replicates in Morus alba. Our study provides the first report of a rubodvirus in Morus alba.

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一种新的白桑猴样病毒的全基因组分析。

桑葚（桑葚属）对养蚕很重要。由于种间杂交和多种栽培品种的存在，许多桑树种的病毒体尚不清楚。在这项研究中，我们鉴定了一种新的负义猴样病毒，并确定了其基因组序列。该病毒具有三个线性单链负链RNA片段：RNA1 （7,329 nt）、RNA2 （1,276 nt）和RNA3 （1,222 nt）。这三个片段中的每一个都包含一个开放阅读框（ORF），分别编码RNA依赖性RNA聚合酶（RdRP）、运动蛋白（MP）和核衣壳蛋白(N)。系统发育分析表明，该病毒属于一个进化支，包括病毒属成员（猴流感病毒科，Hareavirales目，Bunyaviricetes纲），树莓病毒1、葡萄麝香玫瑰病毒和苹果橡胶木病毒2是最近的亲戚。小干扰RNA （siRNA）测序数据表明，该病毒在桑树中复制。本研究首次报道了在桑树中发现的一种鲁伯病毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.