Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-07-29 DOI:10.1007/s00705-025-06373-8

Mantong Zhu, Chang Liu, Guangfeng Liu, Hongsai Zhang, Xing Wang, Ying Wang, Yongqi Luo, Jingzhe Jiang

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Abstract

A Providencia strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its in vitro antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus Kakivirus of the family Autoscriptoviridae.

Graphical abstract

Abstract Image

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从农场废水中分离的一种新型普罗维登斯噬菌体的生物学特性和基因组图谱。

从农场废水中分离出一株普罗维登斯菌株，并将其作为宿主分离出一种名为A06的噬菌体。采用双层琼脂菌斑法确定最佳感染倍数（MOI），构建一步生长曲线，评价噬菌体的温度和pH稳定性，并评价其体外抗菌活性和裂解效率。利用全基因组测序、功能注释和比较基因组学分析对其生物学特征进行分析，确定其分类位置。噬菌体A06具有58个开放阅读框（orf）的环状DNA基因组。确定最佳MOI为100，一步生长曲线显示潜伏期为40 min，裂解期为40 ~ 90 min。A06噬菌体表现出高度的热稳定性和pH耐受性，基因组分析表明它是自转录病毒科卡基病毒属的一个新种。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.