Simultaneous determination of mumefural and citric acid in Japanese apricot fruit juice concentrate using LC with intramolecular excimer-forming derivatization

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2025-07-29 DOI:10.1007/s44211-025-00822-9

Hideyuki Yoshida, Yuka Imura, Sayaka Minematsu, Misaki Ono, Reiko Koga, Hitoshi Nohta

{"title":"Simultaneous determination of mumefural and citric acid in Japanese apricot fruit juice concentrate using LC with intramolecular excimer-forming derivatization","authors":"Hideyuki Yoshida, Yuka Imura, Sayaka Minematsu, Misaki Ono, Reiko Koga, Hitoshi Nohta","doi":"10.1007/s44211-025-00822-9","DOIUrl":null,"url":null,"abstract":"<div><p>Mumefural (MF) which is one of the citrate esters with 5-hydoxymethyl-2-furfural is a component of Japanese apricot fruit juice concentrate (plum extract). A selective LC-fluorescence detection method was developed for the simultaneous quantification of MF and citric acid in plum extracts using intramolecular excimer-forming derivatization. Analytes were derivatized to their corresponding polypyrene derivatives using 4-(1-pyrene)butyric hydrazide (PBH) in the presence of condensation reagent, separated by reversed-phase LC, and detected in the pyrene-excimer fluorescence region (Ex/Em, 345/475 nm). The detection limits (<i>S</i>/<i>N</i> = 3) for the analytes were sub-picomole levels per 10-μL injection volume. The method was applied for the determination of MF and citric acid in plum extracts and a typical plum wine with a simple pretreatment. Large amounts of citric acid were detected in the plum extracts and the plum wine, whereas MF was detected only in the commercial plum extracts.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 9","pages":"1547 - 1553"},"PeriodicalIF":2.0000,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Sciences","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s44211-025-00822-9","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Mumefural (MF) which is one of the citrate esters with 5-hydoxymethyl-2-furfural is a component of Japanese apricot fruit juice concentrate (plum extract). A selective LC-fluorescence detection method was developed for the simultaneous quantification of MF and citric acid in plum extracts using intramolecular excimer-forming derivatization. Analytes were derivatized to their corresponding polypyrene derivatives using 4-(1-pyrene)butyric hydrazide (PBH) in the presence of condensation reagent, separated by reversed-phase LC, and detected in the pyrene-excimer fluorescence region (Ex/Em, 345/475 nm). The detection limits (S/N = 3) for the analytes were sub-picomole levels per 10-μL injection volume. The method was applied for the determination of MF and citric acid in plum extracts and a typical plum wine with a simple pretreatment. Large amounts of citric acid were detected in the plum extracts and the plum wine, whereas MF was detected only in the commercial plum extracts.

Graphical abstract

Abstract Image

查看原文本刊更多论文

分子内模拟衍生液相色谱法同时测定日本杏浓缩汁中的乳酸菌和柠檬酸。

枸橼酸酯（MF）是一种含有5-羟基甲基-2-糠醛的柠檬酸酯，是日本杏浓缩果汁（李子提取物）的主要成分。建立了分子内模拟衍生法同时定量测定李子提取物中MF和柠檬酸的选择性lc -荧光检测方法。在缩合剂存在下，用4-（1-芘）丁基肼（PBH）将分析物衍生为相应的芘衍生物，用反相LC分离，在芘准分子荧光区（Ex/Em, 345/475 nm）检测。检出限（S/N = 3）为亚皮摩尔/ 10 μ l。采用简单的前处理方法测定了李子提取物和典型李子酒中MF和柠檬酸的含量。枸杞提取物和枸杞酒中检测到大量的柠檬酸，而枸杞提取物中仅检测到枸杞酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.