Community-Engaged Course-Based Undergraduate Research of Multidrug Resistance in Escherichia coli in Water Near Dairy and Hog Farms in Michigan

IF 2.7 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2025-07-30 DOI:10.1111/1758-2229.70151

Renee Chowdhry, Soeun Jun, Yuwei Kong, Adrian Casillas Saenz, Jonathan Chung, Michelle Chang, Katie Osborn, Yuhui Zhang, Will Bodeau, Nicole Curristan, Brynn Sofro, Soham Ray, Karina Jimenez, Lynn Henning, Cole Dickerson, Salman Jaberi, Clare Delucchi, Jose Reyes Miranda, Adriane Jones, Carol Bascom-Slack, Jennifer A. Jay

{"title":"Community-Engaged Course-Based Undergraduate Research of Multidrug Resistance in Escherichia coli in Water Near Dairy and Hog Farms in Michigan","authors":"Renee Chowdhry, Soeun Jun, Yuwei Kong, Adrian Casillas Saenz, Jonathan Chung, Michelle Chang, Katie Osborn, Yuhui Zhang, Will Bodeau, Nicole Curristan, Brynn Sofro, Soham Ray, Karina Jimenez, Lynn Henning, Cole Dickerson, Salman Jaberi, Clare Delucchi, Jose Reyes Miranda, Adriane Jones, Carol Bascom-Slack, Jennifer A. Jay","doi":"10.1111/1758-2229.70151","DOIUrl":null,"url":null,"abstract":"<p>Antimicrobial resistance (AMR) was assessed in Michigan surface waters impacted by dairies, swine farms and human wastewater, as well as in an unimpacted (UI) comparison site. <i>Escherichia coli</i> (EC) was quantified in the presence and absence of cefotaxime, as extended-spectrum beta-lactamase-producing EC (ESBL-EC) has been deemed a proxy for AMR. Purified isolates of EC selected without antibiotics were characterised by disk diffusion; antibiotics tested included ampicillin, tetracycline, cefotaxime, cefoxitin, streptomycin, nalidixic acid, kanamycin, ciprofloxacin and erythromycin. Ampicillin and tetracycline resistance ranged up to 67% and 62% of the EC isolates, respectively, at livestock-impacted sites, but were low at UI. Multidrug resistance (MDR) was not observed at all at UI but was observed in up to 76% and 67% of isolates from dairy and swine/dairy, respectively. AMP-TE-E was the most common resistance pattern observed, with all isolates originating from one of the dairy sites. Notably, resistance to cefotaxime did not correlate with MDR, indicating that preselection for ESBL-EC before further AMR testing will not successfully characterise AMR or MDR from culturable EC. Interestingly, the percent of isolates resistant to AMP correlated quite well with MDR. This work highlights the importance of MDR characterisation at livestock-impacted surface water sites.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70151","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.70151","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Antimicrobial resistance (AMR) was assessed in Michigan surface waters impacted by dairies, swine farms and human wastewater, as well as in an unimpacted (UI) comparison site. Escherichia coli (EC) was quantified in the presence and absence of cefotaxime, as extended-spectrum beta-lactamase-producing EC (ESBL-EC) has been deemed a proxy for AMR. Purified isolates of EC selected without antibiotics were characterised by disk diffusion; antibiotics tested included ampicillin, tetracycline, cefotaxime, cefoxitin, streptomycin, nalidixic acid, kanamycin, ciprofloxacin and erythromycin. Ampicillin and tetracycline resistance ranged up to 67% and 62% of the EC isolates, respectively, at livestock-impacted sites, but were low at UI. Multidrug resistance (MDR) was not observed at all at UI but was observed in up to 76% and 67% of isolates from dairy and swine/dairy, respectively. AMP-TE-E was the most common resistance pattern observed, with all isolates originating from one of the dairy sites. Notably, resistance to cefotaxime did not correlate with MDR, indicating that preselection for ESBL-EC before further AMR testing will not successfully characterise AMR or MDR from culturable EC. Interestingly, the percent of isolates resistant to AMP correlated quite well with MDR. This work highlights the importance of MDR characterisation at livestock-impacted surface water sites.

Abstract Image

查看原文本刊更多论文

基于社区参与课程的本科生对密歇根州奶牛场和养猪场附近水体中大肠杆菌多药耐药性的研究

评估了受奶牛场、养猪场和人类废水影响的密歇根州地表水以及未受影响的比较点的抗菌素耐药性（AMR）。在头孢噻肟存在和不存在的情况下，大肠杆菌（EC）被量化，因为广谱β -内酰胺酶产生EC （ESBL-EC）被认为是AMR的代理。在不使用抗生素的情况下，采用圆盘扩散法对大肠杆菌分离物进行鉴定；测试的抗生素包括氨苄西林、四环素、头孢噻肟、头孢西丁、链霉素、萘啶酸、卡那霉素、环丙沙星和红霉素。在家畜疫区，对氨苄西林和四环素的耐药性分别高达67%和62%，但在尿路感染区较低。在UI中完全没有观察到多药耐药（MDR），但分别在乳制品和猪/乳制品中高达76%和67%的分离株中观察到多药耐药。AMP-TE-E是观察到的最常见的耐药模式，所有分离株均来自同一奶牛场。值得注意的是，对头孢他肟的耐药性与耐多药无关，这表明在进一步的AMR检测之前预选ESBL-EC将无法成功地从可培养的EC中确定AMR或MDR。有趣的是，对AMP耐药的分离株百分比与耐多药相当相关。这项工作强调了在受牲畜影响的地表水场址进行多药耐药表征的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.