Atmospheric Cold Plasma Technology Enabling Scalable Surface-Independent Protein Immobilization for Biosensing Applications

IF 4.4 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Lieze Dankers, Bernard Nisol, Derick Yongabi, Tom Van der Donck, Jesús Gándara Loe, Patrick Wagner, Jin Won Seo, Rob Ameloot, Karen Leirs, Jeroen Lammertyn
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Abstract

Biosensors show great potential across various fields including, but not limited to, medical diagnostics, drug development, and environmental monitoring. Yet, commercialization faces challenges, particularly in fabrication and biofunctionalization, due to specific surface properties needed for each application. This highlights the need for a standardized biomolecule immobilization process, enabling straightforward target detection on various surfaces. Cold atmospheric plasma technology offers a scalable solution, combining surface activation with molecule grafting in a single step. This technology is employed to construct stable surface-independent carboxylic acid (COOH) linker-layer coatings, enabling covalent protein immobilization via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry and creating a robust biointerface for bioassay integration. The coating's composition, surface energy, thickness, topography, and stability confirm a secure COOH-rich layer. Biofunctionalization is studied in depth by immobilizing immunoglobulin G (IgG), streptavidin, and protein G. Enzyme-linked immunosorbent assay (ELISA)-based model bioassays demonstrate protein-independent functionalization and linker-layer stability of at least one month (stored in air). The calibration curve for IgG-biotin detection shows a high signal-to-noise ratio. Consistent performance across polymethylmethacrylate (PMMA), cyclic olefin copolymer (COC), polyvinyl chloride (PVC), and glass proves the method’s universal applicability. Hence, this technology enables versatile, scalable, cost-effective biosensor fabrication with high-performance bioreceptor layers on various surfaces.

Abstract Image

大气冷等离子体技术使生物传感应用的可扩展的表面无关蛋白固定化
生物传感器在各个领域显示出巨大的潜力,包括但不限于医疗诊断、药物开发和环境监测。然而,由于每种应用需要特定的表面特性,商业化面临挑战,特别是在制造和生物功能化方面。这突出了对标准化生物分子固定过程的需求,从而能够在各种表面上直接检测目标。冷大气等离子体技术提供了一种可扩展的解决方案,将表面活化与分子接枝在一个步骤中结合起来。该技术用于构建稳定的表面无关羧酸(COOH)连接层涂层,通过1-乙基-3-(3-二甲氨基丙基)碳二亚胺(EDC)化学实现共价蛋白的固定,并为生物测定整合创造了强大的生物界面。涂层的成分、表面能、厚度、形貌和稳定性证实了涂层是一层安全的富含cooh的涂层。通过固定免疫球蛋白G (IgG)、链亲和素和蛋白G,深入研究了生物功能化。基于酶联免疫吸附试验(ELISA)的模型生物测定证明了与蛋白质无关的功能化和连接层稳定性至少一个月(在空气中储存)。igg -生物素检测的校准曲线具有较高的信噪比。该方法在聚甲基丙烯酸甲酯(PMMA)、环烯烃共聚物(COC)、聚氯乙烯(PVC)和玻璃上的性能一致,证明了该方法的普遍适用性。因此,该技术使多功能,可扩展,具有成本效益的生物传感器制造在各种表面上具有高性能的生物受体层。
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来源期刊
Advanced Materials Interfaces
Advanced Materials Interfaces CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
8.40
自引率
5.60%
发文量
1174
审稿时长
1.3 months
期刊介绍: Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018. The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface. Advanced Materials Interfaces covers all topics in interface-related research: Oil / water separation, Applications of nanostructured materials, 2D materials and heterostructures, Surfaces and interfaces in organic electronic devices, Catalysis and membranes, Self-assembly and nanopatterned surfaces, Composite and coating materials, Biointerfaces for technical and medical applications. Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.
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