Lieze Dankers, Bernard Nisol, Derick Yongabi, Tom Van der Donck, Jesús Gándara Loe, Patrick Wagner, Jin Won Seo, Rob Ameloot, Karen Leirs, Jeroen Lammertyn
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引用次数: 0
Abstract
Biosensors show great potential across various fields including, but not limited to, medical diagnostics, drug development, and environmental monitoring. Yet, commercialization faces challenges, particularly in fabrication and biofunctionalization, due to specific surface properties needed for each application. This highlights the need for a standardized biomolecule immobilization process, enabling straightforward target detection on various surfaces. Cold atmospheric plasma technology offers a scalable solution, combining surface activation with molecule grafting in a single step. This technology is employed to construct stable surface-independent carboxylic acid (COOH) linker-layer coatings, enabling covalent protein immobilization via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry and creating a robust biointerface for bioassay integration. The coating's composition, surface energy, thickness, topography, and stability confirm a secure COOH-rich layer. Biofunctionalization is studied in depth by immobilizing immunoglobulin G (IgG), streptavidin, and protein G. Enzyme-linked immunosorbent assay (ELISA)-based model bioassays demonstrate protein-independent functionalization and linker-layer stability of at least one month (stored in air). The calibration curve for IgG-biotin detection shows a high signal-to-noise ratio. Consistent performance across polymethylmethacrylate (PMMA), cyclic olefin copolymer (COC), polyvinyl chloride (PVC), and glass proves the method’s universal applicability. Hence, this technology enables versatile, scalable, cost-effective biosensor fabrication with high-performance bioreceptor layers on various surfaces.
期刊介绍:
Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018.
The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface.
Advanced Materials Interfaces covers all topics in interface-related research:
Oil / water separation,
Applications of nanostructured materials,
2D materials and heterostructures,
Surfaces and interfaces in organic electronic devices,
Catalysis and membranes,
Self-assembly and nanopatterned surfaces,
Composite and coating materials,
Biointerfaces for technical and medical applications.
Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.