Assessing the in vitro anticoagulant and free radical scavenging ability of heparin like polysaccharide from gladius of Doryteuthis sibogae (Adam, 1954)

Pharmacological Research - Natural Products Pub Date : 2025-07-29 DOI:10.1016/j.prenap.2025.100327

Dhivyashri Senthilkumar , Aayisha Aathila Saleem , Suresh Sasikala , Namasivayam Subhapradha , Pasiyappazham Ramasamy

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Abstract

This study focused on extracting heparin-like polysaccharide (HLP) from the gladius of Doryteuthis sibogae and evaluating its anticoagulant and antioxidant potential. The structural characterization of extract was studied using FTIR, FESEM and XRD and the presence of HLP was confirmed. The anticoagulant properties of HLP were studied using the APTT and PT kits. In addition, antioxidant evaluation was performed using DPHH, Hydroxyl radical scavenging assay, and the chelating ability of ferrous ions. The HLP showed anticoagulant efficacy of 6.72 IU/mg and 1.69 IU/mg for APTT and PT assay respectively. HLP extracted from D. sibogae demonstrated antioxidant properties comparable to those of the control substances used in various tests. The increase in the concentration of the polysaccharide extract was directly proportional to the antioxidant properties elicited by the HLP of D. sibogae. Finally, HLP extracted from D. sibogae showed significant anticoagulant and free radical-scavenging activities.

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唐菖蒲类肝素多糖体外抗凝血和清除自由基能力的研究（Adam, 1954）

本研究主要从龙舌兰中提取肝素样多糖（HLP），并评价其抗凝血和抗氧化活性。利用FTIR、FESEM和XRD对提取物进行了结构表征，并证实了HLP的存在。采用APTT和PT试剂盒研究了HLP的抗凝血性能。此外，还通过DPHH、羟基自由基清除试验和铁离子螯合能力对其抗氧化性进行了评价。APTT和PT的抗凝效果分别为6.72 IU/mg和1.69 IU/mg。从黄芪中提取的高效液相色谱显示出与各种试验中使用的对照物质相当的抗氧化性能。多糖提取物浓度的增加与黄芪高脂多糖的抗氧化作用成正比。结果表明，黄芪提取物具有明显的抗凝血和自由基清除活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pharmacological Research - Natural Products

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