The dual symbiosis of the belowground arbuscular mycorrhizal fungus and aboveground Epichloë endophyte synergistically alleviates perennial ryegrass leaf spots caused by Bipolaris sorokiniana

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-07-28 DOI:10.1016/j.pmpp.2025.102856

Fang Li , Jie Deng , Youlei Shen , Tingyu Duan , Chao Xia

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引用次数: 0

Abstract

Arbuscular mycorrhizal fungi (AMF) and Epichloë endophyte are two common symbiotic fungi that enhance plant resistance to stress, but their combined effect on plant responses to biotic stress remains unclear. Using RNA-seq techniques, we evaluated the impact of co-colonization of AMF Claroideoglomus etunicatum and Epichloë festucae var. lolii endophyte on perennial ryegrass (Lolium perenne) response to leaf spot disease caused by Bipolaris sorokiniana. Disease severity, phytohormones, analyzed the differential expression genes related to pathogen defence to perennial ryegrass with or without AMF and Epichloë were evaluated. The co-colonization of AMF and Epichloë reduced leaf spot severity, increased plant defence enzymes, particularly catalase (CAT) and polyphenol oxidase (PPO) in diseased plants. The co-colonization of AMF and Epichloë regulated plant disease resistance genes related to terpenoid backbone biosynthesis, biotin metabolism, and plant-pathogen interaction. Weighted Gene Co-Expression Network Analysis (WGCNA) identified 27 hub genes and 11 resistance-related genes specifically associated with the co-colonization. These findings enhance our understanding of the molecular mechanisms underlying the AMF-Epichloë synergy and their combined effects on perennial ryegrass resistance to leaf spot disease, thereby facilitating the utilization of symbiotic microorganisms in plant disease management.

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地下丛枝菌根真菌与地上Epichloë内生菌的双重共生，协同缓解了黑麦草多年生叶斑病

丛枝菌根真菌（AMF）和Epichloë内生真菌是两种常见的增强植物抗逆性的共生真菌，但它们对植物生物胁迫反应的共同作用尚不清楚。利用RNA-seq技术，研究了黑麦草内生真菌（AMF）和Epichloë festucae var. lolii共定殖对黑麦草（Lolium perenne）对双极星（Bipolaris sorokiniana）叶斑病的响应。对多年生黑麦草有或无AMF时的疾病严重程度、植物激素、与病原菌防御相关的差异表达基因和Epichloë进行了评价。AMF和Epichloë的共定殖降低了病株的叶斑病严重程度，增加了植物防御酶，特别是过氧化氢酶（CAT）和多酚氧化酶（PPO）。AMF和Epichloë的共定殖调节了与萜类主干生物合成、生物素代谢和植物-病原体相互作用相关的植物抗病基因。加权基因共表达网络分析（WGCNA）鉴定出27个中心基因和11个与共定植特异性相关的抗性相关基因。这些发现加深了我们对AMF-Epichloë协同作用及其对多年生黑麦草抗叶斑病的综合作用的分子机制的理解，从而促进了共生微生物在植物病害管理中的利用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.