A collagen-based amniotic membrane scaffold combined with photobiomodulation accelerates wound repair in diabetic rats through modulation of inflammation and tissue regeneration

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-07-29 DOI:10.1016/j.tice.2025.103063

Ahmed Hjazi

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引用次数: 0

Abstract

Chronic diabetic wounds present significant challenges to effective tissue repair due to persistent inflammation and impaired regeneration. In this study, we investigated the therapeutic potential of a bioengineered collagen scaffold derived from the human amniotic membrane (CSAM), used alone or in combination with photobiomodulation therapy (PBMT), in a diabetic rat wound model. Forty diabetic rats were allocated into four groups: control, CSAM, PBMT, and CSAM+PBMT. Wound healing was assessed on days 4 and 8 post-injury. Combined treatment (CSAM+PBMT) significantly enhanced wound closure and histological regeneration, with notable increases in fibroblast and blood vessel density, epidermal and dermal thickness, collagen deposition, and expression levels of VEGF and bFGF compared to other groups. Additionally, this group exhibited a marked reduction in neutrophil infiltration and pro-inflammatory cytokines, including IL-1β, TNF-α, and NF-κB. These results demonstrate that the synergistic application of PBMT and CSAM fosters a pro-regenerative wound microenvironment by suppressing inflammation and promoting cellular proliferation and extracellular matrix remodeling. This combinatory approach offers a promising therapeutic avenue for improving diabetic wound healing outcomes.

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胶原基羊膜支架联合光生物调节通过调节炎症和组织再生加速糖尿病大鼠伤口修复

由于持续的炎症和再生受损，慢性糖尿病伤口对有效的组织修复提出了重大挑战。在这项研究中，我们研究了来自人羊膜（CSAM）的生物工程胶原支架单独使用或与光生物调节疗法（PBMT）联合使用在糖尿病大鼠伤口模型中的治疗潜力。将40只糖尿病大鼠分为对照组、CSAM组、PBMT组和CSAM+PBMT组。于伤后第4天和第8天评估伤口愈合情况。CSAM+PBMT联合治疗（CSAM+PBMT）显著促进创面愈合和组织再生，与其他组相比，成纤维细胞和血管密度、表皮和真皮厚度、胶原沉积、VEGF和bFGF表达水平均显著增加。此外，该组中性粒细胞浸润和促炎细胞因子（包括IL-1β、TNF-α和NF-κB）明显减少。这些结果表明，PBMT和CSAM的协同应用通过抑制炎症、促进细胞增殖和细胞外基质重塑来培养促再生的伤口微环境。这种联合方法为改善糖尿病伤口愈合提供了一种有希望的治疗途径。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.