In vitro production of bioactive compounds and antioxidant potential in leaf-derived callus cultures of Paeonia emodi Royle

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-07-31 DOI:10.1016/j.bse.2025.105101

Kuldeep Joshi , Priyanka Adhikari , Arun Jugran , Indra D. Bhatt

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Abstract

This study assessed phenolic compound levels and antioxidant activity in Paeonia emodi Royle leaf callus tissue produced in vitro, offering a sustainable alternative to wild-harvested plant material. The callus of P. emodi was initiated on Murashige and Skoog (MS) medium supplemented with 0.5 μM naphthylacetic acid (NAA) and 0.5 μM thidiazuron (TDZ). Maximum callus biomass (1.54 ± 0.08 g) was observed in the 4th week of culture. Samples were extracted using four different solvents: methanol, ethanol, acetone, and ethyl acetate. Among these, the methanolic extract showed higher levels of phytochemicals and antioxidant activity. Although total phenolic (16.42 ± 0.23 mg GAE/g dw), flavonol (8.30 ± 0.13 mg QE/g dw), and tannin (7.17 ± 0.09 mg TAE/g dw) were highest in the 4th week, total flavonoid (9.20 ± 0.03 mg QE/g dw) peaked in the 5th week. HPLC analysis revealed maximum emodin (0.221 ± 0.010 mg/g dw) in the 4th week and paeoniflorin (0.573 ± 0.003 mg/g dw) in the 5th week. The 4th week methanolic extract showed the strongest antioxidant activity in ABTS, DPPH, and FRAP assays, with IC₅₀ values significantly lower than other harvests. The findings demonstrate that in vitro callus cultures of P. emodi can serve as a potential alternative source for bioactive compounds, reducing dependence on wild populations and offering applications in the pharmaceutical and nutraceutical sectors and industries.

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芍药叶源愈伤组织生物活性物质的体外产生及抗氧化能力研究

本研究评估了离体生产的芍药叶愈伤组织中酚类化合物水平和抗氧化活性，为野生收获的植物材料提供了可持续的替代品。在添加0.5 μM萘乙酸（NAA）和0.5 μM硫代脲（TDZ）的Murashige和Skoog （MS）培养基上诱导黄芪愈伤组织。培养第4周愈伤组织生物量最大（1.54±0.08 g）。样品用甲醇、乙醇、丙酮和乙酸乙酯四种不同的溶剂提取。其中，甲醇提取物显示出较高的植物化学物质水平和抗氧化活性。总酚（16.42±0.23 mg QE/g dw）、黄酮醇（8.30±0.13 mg QE/g dw）和单宁（7.17±0.09 mg TAE/g dw）在第4周最高，总黄酮（9.20±0.03 mg QE/g dw）在第5周达到峰值。HPLC分析显示，第4周大黄素含量最高（0.221±0.010 mg/g dw），第5周芍药苷含量最高（0.573±0.003 mg/g dw）。第4周甲醇提取物在ABTS、DPPH和FRAP试验中表现出最强的抗氧化活性，IC50值显著低于其他收获。研究结果表明，该菌株的愈伤组织培养物可以作为生物活性化合物的潜在替代来源，减少对野生种群的依赖，并在制药和营养保健部门和工业中提供应用。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.