Individual variations in buffalo somatic cell cloning efficiency: Synergistic regulation by mitochondria and chromatin remodeling

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-07-25 DOI:10.1016/j.theriogenology.2025.117602

Xiaofen Yang , Haoying Gan , Yuwei Bai , Tingzi Yu , Dongping Zhou , Yun Wang , Ruru Jia , Chunye Wei , Mingzhe Lu , Fenghua Lu

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Abstract

The molecular mechanisms underlying differences in somatic cell cloning efficiency among buffalo fetal fibroblasts (BFFs) from different individuals remain poorly understood. Our study conducted a comparative analysis of mitochondrial function and chromatin remodeling capacity of BFFs from different individuals, and tracked the mitochondria in the embryos derived from SCNT and IVF. These findings revealed that BFFs with high cloning efficiency displayed well-preserved mitochondrial morphology and ultrastructure, increased mitochondrial DNA copy number and ATP levels, elevated antioxidant capacity, enhanced mitochondrial membrane potential, and significantly upregulated expression levels of mitochondria-related genes. Meanwhile, BFFs with high cloning efficiency demonstrated significantly higher DNA enrichment, lower heterochromatin levels, and differential expression levels of chromatin remodeling-related genes. Furthermore, the persistence of donor cell mitochondria during cloned embryo development was observed, whereas sperm-derived mitochondria during IVF embryo development were rarely detectable. Collectively, our results suggest a tight connection between the mitochondria and chromatin remodeling of donor cells, and demonstrating their synergistic impact on cloning efficiency, providing the crucial experimental evidence for nucleo-mitochondrial interactions.

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水牛体细胞克隆效率的个体差异：线粒体和染色质重塑的协同调节

不同个体水牛胎儿成纤维细胞体细胞克隆效率差异的分子机制尚不清楚。本研究对不同个体的闺蜜的线粒体功能和染色质重塑能力进行了比较分析，并对SCNT和体外受精胚胎的线粒体进行了追踪。结果表明，克隆效率高的闺蜜线粒体形态和超微结构保存较好，线粒体DNA拷贝数和ATP水平增加，抗氧化能力增强，线粒体膜电位增强，线粒体相关基因表达水平显著上调。同时，克隆效率高的闺蜜DNA富集程度高，异染色质水平低，染色质重塑相关基因表达差异明显。此外，在克隆胚胎发育过程中观察到供体细胞线粒体的持久性，而在体外受精胚胎发育过程中很少检测到精子来源的线粒体。总之，我们的研究结果表明，线粒体和供体细胞的染色质重塑之间存在紧密联系，并证明了它们对克隆效率的协同影响，为核-线粒体相互作用提供了重要的实验证据。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.