{"title":"Preclinical assessment of agreement between optical frequency domain imaging and pathology in rabbit subglottic mucosal lesions","authors":"Yuki Komyo , Yuichiro Tomioka , Yuichi Okata , Harunori Miyauchi , Taichi Nakatani , Shino Inuzuka , Tomoo Itoh , Yuko Bitoh","doi":"10.1016/j.ijporl.2025.112499","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The pediatric subglottic airway is susceptible to various pathological conditions, both congenital and acquired. Accurate imaging of subglottic mucosal lesions is essential for appropriate treatments, but existing methods such as bronchoscopy or computed tomography (CT) often lack resolution or objectivity. Optical coherence tomography (OCT) has been used, but the correlation between OCT-based imaging and histopathological findings has not been evaluated. Optical frequency domain imaging (OFDI), a clinical-grade variant of OCT, offers higher resolution and faster acquisition. We aimed to evaluate whether OFDI can safely and clearly visualize subglottic mucosal structures and examine how well the imaging findings correspond to histological features in an animal model.</div></div><div><h3>Methods</h3><div>Twelve rabbits weighing approximately 3 kg were used. Under anesthesia, brush scrubbing was performed to induce mucosal lesions in the subglottic space. The resected larynx was pathologically examined. The primary efficacy endpoints were circumferential visualization of the subglottic space and concordance with pathology in differentiating between the cartilage, mucosa, and lesions. The primary safety endpoints were the absence of mucosal injury and catheter dislodgement during OFDI.</div></div><div><h3>Results</h3><div>Clear images of the subglottic space were obtained, and OFDI clearly distinguished the cartilage from the mucosa and lesions. The interobserver reliability of the OCT assessment was 0.87, and the agreement between OCT and the histological assessment was 0.94. No mucosal hemorrhage or catheterization-induced dehiscence was observed.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that OFDI can be used safely and has a diagnostic performance comparable to that of histological assessment of mucosal lesions in the subglottic space.</div></div>","PeriodicalId":14388,"journal":{"name":"International journal of pediatric otorhinolaryngology","volume":"196 ","pages":"Article 112499"},"PeriodicalIF":1.3000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of pediatric otorhinolaryngology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165587625002861","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
The pediatric subglottic airway is susceptible to various pathological conditions, both congenital and acquired. Accurate imaging of subglottic mucosal lesions is essential for appropriate treatments, but existing methods such as bronchoscopy or computed tomography (CT) often lack resolution or objectivity. Optical coherence tomography (OCT) has been used, but the correlation between OCT-based imaging and histopathological findings has not been evaluated. Optical frequency domain imaging (OFDI), a clinical-grade variant of OCT, offers higher resolution and faster acquisition. We aimed to evaluate whether OFDI can safely and clearly visualize subglottic mucosal structures and examine how well the imaging findings correspond to histological features in an animal model.
Methods
Twelve rabbits weighing approximately 3 kg were used. Under anesthesia, brush scrubbing was performed to induce mucosal lesions in the subglottic space. The resected larynx was pathologically examined. The primary efficacy endpoints were circumferential visualization of the subglottic space and concordance with pathology in differentiating between the cartilage, mucosa, and lesions. The primary safety endpoints were the absence of mucosal injury and catheter dislodgement during OFDI.
Results
Clear images of the subglottic space were obtained, and OFDI clearly distinguished the cartilage from the mucosa and lesions. The interobserver reliability of the OCT assessment was 0.87, and the agreement between OCT and the histological assessment was 0.94. No mucosal hemorrhage or catheterization-induced dehiscence was observed.
Conclusions
Our findings suggest that OFDI can be used safely and has a diagnostic performance comparable to that of histological assessment of mucosal lesions in the subglottic space.
期刊介绍:
The purpose of the International Journal of Pediatric Otorhinolaryngology is to concentrate and disseminate information concerning prevention, cure and care of otorhinolaryngological disorders in infants and children due to developmental, degenerative, infectious, neoplastic, traumatic, social, psychiatric and economic causes. The Journal provides a medium for clinical and basic contributions in all of the areas of pediatric otorhinolaryngology. This includes medical and surgical otology, bronchoesophagology, laryngology, rhinology, diseases of the head and neck, and disorders of communication, including voice, speech and language disorders.