Anion-Driven Influence of Tetrabutylammonium-Based Ionic Liquids on DNA Stability and Interaction Mechanisms

IF 4.3 Q2 CHEMISTRY, PHYSICAL

ACS Physical Chemistry Au Pub Date : 2025-04-22 DOI:10.1021/acsphyschemau.5c00015

K. K. Athira, and , Ramesh L. Gardas*,

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引用次数: 0

Abstract

Ionic liquids (ILs) have been widely used as alternative solvents for the stabilization, storage, and extraction of DNA. However, studies on the interaction between ammonium-based ILs and DNA, particularly focusing on the effect of anions, remain limited. Tetrabutylammonium (TBA) cation-based ILs with propanoate, bromide, glutamate, and threoninate anions were used to analyze IL–DNA interactions through UV–vis titrations, steady-state and time-resolved fluorescence, and molecular docking. The conformational stability and thermal stability of DNA in IL solutions were analyzed through circular dichroism spectroscopy and UV thermal studies, respectively. Viscosity measurements of the IL solutions were carried out to support the data obtained from UV thermal studies. The TBA cation displays multiple modes of interaction at the groove through electrostatic, hydrophobic, and hydrogen bonding. Among the studied anions, the propanoate anion exhibits significant hydrophobic interactions in addition to hydrogen bonding, whereas glutamate and threoninate primarily engage in hydrogen bonding. The difference in the effect of the ILs on DNA underscores the significant influence of the anions on IL–DNA interactions.

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阴离子驱动的四丁基铵离子液体对DNA稳定性的影响及其相互作用机制。

离子液体作为稳定、储存和提取DNA的替代溶剂已被广泛应用。然而，关于氨基il与DNA相互作用的研究，特别是对阴离子作用的研究仍然有限。通过紫外-可见滴定、稳态和时间分辨荧光以及分子对接，利用四丁基铵（TBA）阳离子与丙酸盐、溴酸盐、谷氨酸盐和苏氨酸盐阴离子的IL-DNA相互作用分析。通过圆二色光谱和紫外热研究分别分析了DNA在IL溶液中的构象稳定性和热稳定性。进行了IL溶液的粘度测量，以支持从紫外热研究中获得的数据。TBA阳离子通过静电、疏水和氢键在凹槽处表现出多种相互作用模式。在研究的阴离子中，丙酸阴离子除氢键外还表现出显著的疏水相互作用，而谷氨酸和苏氨酸主要参与氢键。白细胞介素对DNA影响的差异强调了阴离子对白细胞介素-DNA相互作用的重要影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Physical Chemistry Au 物理化学-

CiteScore

3.70

自引率

0.00%

发文量

期刊介绍： ACS Physical Chemistry Au is an open access journal which publishes original fundamental and applied research on all aspects of physical chemistry. The journal publishes new and original experimental computational and theoretical research of interest to physical chemists biophysical chemists chemical physicists physicists material scientists and engineers. An essential criterion for acceptance is that the manuscript provides new physical insight or develops new tools and methods of general interest. Some major topical areas include:Molecules Clusters and Aerosols; Biophysics Biomaterials Liquids and Soft Matter; Energy Materials and Catalysis