Cetrimide-enhanced Calcium Hydroxide Paste: A Biocompatible and Bioactive Option for Regenerative Endodontics.

IF 3.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2025-07-25 DOI:10.1016/j.joen.2025.07.016

Júlia Eick Iglesias, Lucas Siqueira Pinheiro, Gabriela Cardoso Ferreira, Marla Cuppini, Fabrício Mezzomo Collares, Fabiana Soares Grecca

{"title":"Cetrimide-enhanced Calcium Hydroxide Paste: A Biocompatible and Bioactive Option for Regenerative Endodontics.","authors":"Júlia Eick Iglesias, Lucas Siqueira Pinheiro, Gabriela Cardoso Ferreira, Marla Cuppini, Fabrício Mezzomo Collares, Fabiana Soares Grecca","doi":"10.1016/j.joen.2025.07.016","DOIUrl":null,"url":null,"abstract":"Introduction: This study compared the cytotoxicity, bioactivity, and biocompatibility of calcium hydroxide paste (CHP) with or without 0.2% cetrimide in microspheres (CETm) or in pure form (CET).Methods: CETm or CET were added to CHP and evaluated for cytocompatibility (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay) and bioactivity (Alizarin Red and alkaline phosphatase), using stem cells from the apical papilla. Biocompatibility was assessed by implanting polyethylene tubes containing the materials into rat subcutaneous tissue. One-way ANOVA followed by Tukey post hoc was used to analyze the results of 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide, alkaline phosphatase, and Alizarin Red assays. Kruskal-Wallis with Dunn's post hoc test was used for the biocompatibility test (P < .05).Results: All pastes exhibited cell viability above 100% and bioactivity in alkaline phosphatase and Alizarin Red assays. No abscesses, eosinophils, neutrophils, or giant cells were observed. After 90 days, all materials induced fibrous condensation, and inflammation decreased from mild to insignificant.Conclusion: Adding CETm or CET to CHP may offer a potential intracanal medication for regenerative endodontic procedures, demonstrating favorable biological properties.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of endodontics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.joen.2025.07.016","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: This study compared the cytotoxicity, bioactivity, and biocompatibility of calcium hydroxide paste (CHP) with or without 0.2% cetrimide in microspheres (CETm) or in pure form (CET).

Methods: CETm or CET were added to CHP and evaluated for cytocompatibility (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay) and bioactivity (Alizarin Red and alkaline phosphatase), using stem cells from the apical papilla. Biocompatibility was assessed by implanting polyethylene tubes containing the materials into rat subcutaneous tissue. One-way ANOVA followed by Tukey post hoc was used to analyze the results of 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide, alkaline phosphatase, and Alizarin Red assays. Kruskal-Wallis with Dunn's post hoc test was used for the biocompatibility test (P < .05).

Results: All pastes exhibited cell viability above 100% and bioactivity in alkaline phosphatase and Alizarin Red assays. No abscesses, eosinophils, neutrophils, or giant cells were observed. After 90 days, all materials induced fibrous condensation, and inflammation decreased from mild to insignificant.

Conclusion: Adding CETm or CET to CHP may offer a potential intracanal medication for regenerative endodontic procedures, demonstrating favorable biological properties.

查看原文本刊更多论文

西trimide增强氢氧化钙膏：再生牙髓学的生物相容性和生物活性选择。

前言：本研究比较了添加或不添加0.2%西三酰胺微球（CETm）或纯形式（CET）的氢氧化钙糊（CHP）的细胞毒性、生物活性和生物相容性。方法：在CHP中加入CETm或CETm，用根尖乳头干细胞评价细胞相容性（MTT法）和生物活性（茜素红和碱性磷酸酶）。将聚乙烯管植入大鼠皮下组织，评价其生物相容性。MTT、ALP和ALZ分析结果采用单因素方差分析和Tukey事后分析。采用Kruskal-Wallis和Dunn事后试验进行生物相容性试验(结果：所有膏体在ALP和ALZ试验中均表现出100%以上的细胞活力和生物活性。未见脓肿、嗜酸性粒细胞、中性粒细胞或巨细胞。90 d后，所有材料均发生纤维凝结，炎症由轻度减轻到不明显。结论：将CETm或CET添加到CHP中可能为REPs提供潜在的管内药物，显示出良好的生物学特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.