Pyrogallol Exhibits Antimicrobial Adjuvant Properties Against Staphylococcus aureus and Staphylococcus epidermidis.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-07-28 DOI:10.1093/jambio/lxaf189

Katharina H C Roese, Sarah Ibrahim, Lauren A Cooper, Afnan S Yahya, Dongjae Kang, Nikhil R Tirupathigari, Koby A J LaRose, Karen L Houseknecht, Deborah J Barlow, Kristin M Burkholder

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引用次数: 0

Abstract

Aims: To investigate the effect of pyrogallol as an antimicrobial adjuvant against staphylococci.

Methods and results: We tested the effect of pyrogallol on the susceptibility of four strains of S. aureus and S. epidermidis to antibiotics. In minimum inhibitory concentration (MIC) assays, pyrogallol significantly enhanced the anti-staphylococcal efficacy of linezolid against all strains and increased cefoxitin and oxacillin activity against individual strains. Given pyrogallol's prooxidant properties, we compared its effect on linezolid activity in wild-type and catalase-deficient S. aureus. Catalase-deficient bacteria exhibited greater linezolid sensitivity than WT in presence of pyrogallol. Antibiotic accumulation assays revealed greater intracellular linezolid concentration in S. aureus treated with pyrogallol compared to bacteria treated with linezolid alone.

Conclusions: Pyrogallol potentiates linezolid-mediated killing of staphylococci in a manner involving oxidative stress and intra-bacterial antibiotic accumulation. Pyrogallol may have utility as an anti-staphylococcal antimicrobial adjuvant.

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邻苯三酚具有抗金黄色葡萄球菌和表皮葡萄球菌的抗菌佐剂特性。

目的：研究邻苯三酚作为抗菌佐剂对葡萄球菌的作用。方法与结果：测定邻苯三酚对金黄色葡萄球菌和表皮葡萄球菌4株抗生素敏感性的影响。在最低抑制浓度（MIC）试验中，邻苯三酚显著增强了利奈唑胺对所有菌株的抗葡萄球菌效果，并增加了头孢西丁和恶西林对单个菌株的抗葡萄球菌活性。鉴于邻苯三酚的抗氧化特性，我们比较了其对野生型和过氧化氢酶缺陷金黄色葡萄球菌中利奈唑胺活性的影响。过氧化氢酶缺陷菌在邻苯三酚存在下比WT表现出更高的利奈唑胺敏感性。抗生素积累试验显示，与单独用利奈唑胺处理的细菌相比，用邻苯三酚处理的金黄色葡萄球菌细胞内利奈唑胺浓度更高。结论：邻苯三酚通过氧化应激和细菌内抗生素积累的方式增强了利奈唑胺介导的葡萄球菌杀伤作用。邻苯三酚可作为抗葡萄球菌抗菌佐剂使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.