Peiyao Liu, Chunna Cao, Xiangding Wang, Kewei Li, Ebadu Areb, Ran Li, Qingfeng Zhang, Chuanying Pan, Xianyong Lan
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引用次数: 0
Abstract
Introduction: Bone morphogenetic protein 15 (BMP15) is a pivotal regulator of reproductive performance. This study aimed to investigate genetic variations in the sheep BMP15 gene and their role in lambing traits.
Methods: Whole-genome sequencing was performed on 2,409 individuals from 75 global sheep breeds to identify functional variations in BMP15. Variant annotation, protein structure prediction, haplotype analysis, and association studies with lambing traits were conducted.
Results: 139 SNPs were detected, including 10 exonic variants (6 missense, 2 synonymous, 2 premature stop codons), 9 of which were novel. The high-frequency missense mutation g.54284258A>G (L251P) showed predicted localized conformational changes. Low-frequency stop-gain mutations (e.g., g.54284551C>T) were rare (<0.005%) in domestic breeds. Haplotype H6 (AAAA) predominated in domestic breeds (48.4%), diverging significantly from the wild ancestral H1 (AAGA), indicating artificial selection. The g.54284258A>G variant was significantly associated with live lamb number at first (p < 0.01) and second parity (p < 0.05). AG genotypes had more live lambs than AA (1.29 vs. 1.12; p < 0.01). No significant associations were found with total lambing number, lamb survival rate, stillbirth count, or stillbirth rate.
Discussion: BMP15 exhibits substantial genetic variability shaped by selection. The g.54284258A>G variant significantly influences live lamb numbers and may serve as a genetic marker for improving this trait in sheep breeding programs, though its impact is parity-specific and unrelated to stillbirth or overall survival metrics.
期刊介绍：
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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