Characterization of Vitreous Microbiota Dysbiosis Associated with Proliferative Diabetic Retinopathy.

IF 3 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Fangying Song, Yan Qi, Wenhui Ma, Jun Li, Yan Gao, Xiubin Ma
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引用次数: 0

Abstract

Purpose: Emerging evidence suggests an association between ocular microbiota dysbiosis and ophthalmic diseases; however, the role of the posterior segment microbiome in diabetic retinopathy (DR) remains poorly characterized. In this study, we characterized the vitreous microbiome of patients with proliferative diabetic retinopathy (PDR) and systematically compared its microbial community structure with that of healthy controls.

Methods: A cohort of 19 PDR patients with type 2 diabetes mellitus and 19 non-DR controls were enrolled, with vitreous samples obtained through vitrectomy. Vitreous microbial composition was characterized using 2bRAD-M sequencing technology, enabling species-level taxonomic resolution. The comparison of dominant taxa, biomarker analysis and metabolic pathway differences between the two groups were further explored.

Results: The results of microbiome profiling revealed significant compositional differences in the vitreous core microbiome of PDR patients compared to controls, potentially associated with enhanced activity in membrane transport, nucleotide metabolism and carbohydrate metabolism pathways. LEfSe analysis identified 536 distinctive biomarkers of the two groups. At species level, the PDR group had significantly lower relative abundances of CAG-485_sp009775375, Akkermansia_muciniphila and Bacteroides_acidifaciens, compared with control group.

Conclusion: This is the first study confirming the microbiota in human vitreous fluid samples by 2bRAD-M sequencing. These findings suggest a potential link between vitreous microbial dysbiosis and PDR, offering novel insights for future mechanistic investigations into DR.

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与增殖性糖尿病视网膜病变相关的玻璃体微生物群失调的特征。
目的:新的证据表明,眼部微生物群失调与眼部疾病之间存在关联;然而,后段微生物组在糖尿病视网膜病变(DR)中的作用仍然知之甚少。在这项研究中,我们对增殖性糖尿病视网膜病变(PDR)患者的玻璃体微生物群进行了表征,并系统地将其微生物群落结构与健康对照进行了比较。方法:纳入19例合并2型糖尿病的PDR患者和19例非dr对照组,通过玻璃体切除术获得玻璃体样本。利用2bRAD-M测序技术对玻璃体微生物组成进行了表征,实现了物种水平的分类分辨率。进一步探讨两组间优势类群比较、生物标志物分析及代谢途径差异。结果:微生物组分析结果显示,与对照组相比,PDR患者玻璃体核心微生物组的组成存在显著差异,这可能与膜转运、核苷酸代谢和碳水化合物代谢途径的活性增强有关。LEfSe分析鉴定出两组的536个不同的生物标志物。在种水平上,PDR组CAG-485_sp009775375、Akkermansia_muciniphila和bacteroides_acidifacens的相对丰度显著低于对照组。结论:本研究首次通过2bRAD-M测序方法确定了人玻璃体液样品中的微生物群。这些发现表明玻璃体微生物生态失调与PDR之间存在潜在联系,为未来DR的机制研究提供了新的见解。
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来源期刊
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
5.90
自引率
6.10%
发文量
431
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed, open access, online journal. The journal is committed to the rapid publication of the latest laboratory and clinical findings in the fields of diabetes, metabolic syndrome and obesity research. Original research, review, case reports, hypothesis formation, expert opinion and commentaries are all considered for publication.
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