In Vitro Hepatocyte Differentiation of Human Adipose-Derived Stem Cells Under Hypoxia and Photobiomodulation Irradiation.

IF 1.7 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cellular reprogramming Pub Date : 2025-10-01 Epub Date: 2025-07-28 DOI:10.1177/21524971251363134
In-Su Park
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Abstract

Stem cells may be manipulated in vitro to induce hepatic differentiation. We investigated the effect of hypoxia and photobiomodulation therapy (PBMT) on the hepatogenic differentiation of human adipose-derived stem cells (hASCs). hASCs were exposed to different carbon dioxide concentrations with photobiomodulation (PBM) using low-level light. Cell survival and secretion of hepatocyte growth factor (HGF) of the hASCs were evaluated by immunostaining and Western blot analyses. Hepatic differentiation was assessed via immunocytochemical staining, fluorescence-activated cell sorting, and Western blot analysis for liver-specific genes and proteins, including albumin (ALB), cytokeratins 8/18, and alpha-fetoprotein (AFP). PBM therapy has been shown to enhance proliferation and cytokine secretion of a number of cells. The expression profiles of ALB, AFP, and cytokeratin 8/18 demonstrated that when HGF, hypoxia, or PBMT were treated individually, incomplete hepatocyte differentiation was achieved. In contrast, quantitative analysis of ALB, cytokeratins 8/18, and AFP showed that HGF was enhanced significantly by hypoxia+PBM treatment. The obtained cell populations contained progenitors that expressed both hepatic ALB and cytokeratin 8/18 markers, as well as AFP. These data suggest that PBMT and hypoxia are effective biostimulators of hASCs in hepatogenic differentiation, which enhances the survival of hASCs and stimulates the secretion of growth factors.

缺氧和光生物调节照射下人脂肪干细胞体外肝细胞分化的研究。
干细胞可以在体外诱导肝脏分化。我们研究了缺氧和光生物调节疗法(PBMT)对人脂肪源性干细胞(hASCs)肝源性分化的影响。利用低强度光进行光生物调节(PBM),将hASCs暴露于不同浓度的二氧化碳中。采用免疫染色法和Western blot法检测细胞存活率和肝细胞生长因子(HGF)分泌情况。通过免疫细胞化学染色、荧光活化细胞分选和肝脏特异性基因和蛋白质的Western blot分析来评估肝脏分化,包括白蛋白(ALB)、细胞角蛋白8/18和甲胎蛋白(AFP)。PBM治疗已被证明可以增强许多细胞的增殖和细胞因子的分泌。ALB、AFP和细胞角蛋白8/18的表达谱表明,当HGF、缺氧或PBMT单独处理时,肝细胞分化不完全。相比之下,定量分析ALB、细胞角蛋白8/18和AFP显示,缺氧+PBM治疗显著增强HGF。获得的细胞群包含表达肝脏ALB和细胞角蛋白8/18标记物以及AFP的祖细胞。这些数据表明,PBMT和缺氧是hASCs在肝源性分化过程中有效的生物刺激剂,可以提高hASCs的存活率,刺激生长因子的分泌。
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来源期刊
Cellular reprogramming
Cellular reprogramming CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CiteScore
2.50
自引率
6.20%
发文量
37
审稿时长
3 months
期刊介绍: Cellular Reprogramming is the premier journal dedicated to providing new insights on the etiology, development, and potential treatment of various diseases through reprogramming cellular mechanisms. The Journal delivers information on cutting-edge techniques and the latest high-quality research and discoveries that are transforming biomedical research. Cellular Reprogramming coverage includes: Somatic cell nuclear transfer and reprogramming in early embryos Embryonic stem cells Nuclear transfer stem cells (stem cells derived from nuclear transfer embryos) Generation of induced pluripotent stem (iPS) cells and/or potential for cell-based therapies Epigenetics Adult stem cells and pluripotency.
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