Transcript PHF19-207 May Be a Long Non-Coding RNA with Tumor-Promoting Role in Colon Cancer.

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2025-07-02 DOI:10.3390/biom15070957

Dunja Pavlovic, Tamara Babic, Sofija Ignjatovic, Katarina Pavlovic, Sandra Dragicevic, Aleksandra Nikolic

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引用次数: 0

Abstract

Recent pan-cancer transcriptome analysis has revealed differential activity of two alternative PHF19 gene promoters in malignant versus non-malignant gut mucosa. One of these promoters upregulated in colon cancer leads to the expression of the PHF19-207 transcript, suggesting its potential role in tumor promotion. The objective of this study was to investigate the function of PHF19-207 using in silico tools and publicly available data, as well as to assess its expression in colon cancer. Expression analyses were conducted via qPCR and RNA sequencing on RNA extracted from the immortalized colonic epithelial cell line HCEC-1CT, as well as a series of colon cancer cell lines cultured in both 2D and 3D environments. The expression of PHF19-207 was found to be elevated in all malignant cell lines compared to the non-malignant HCEC-1CT cell line in both culture conditions, with the most prominent increase observed in cell lines derived from advanced stages of the disease and in the HCEC-1CT cell line overexpressing KRAS. Furthermore, the PHF19-207 transcript was detected in exosomes derived from malignant cells. These findings suggest that PHF19-207 holds potential as a diagnostic biomarker. In addition, in silico analyses indicate that this transcript may function as a long non-coding RNA involved in the regulation of gene expression. Further functional investigations are required to elucidate its precise role in colon carcinogenesis.

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转录物PHF19-207可能是结肠癌中具有促肿瘤作用的长链非编码RNA。

最近的泛癌转录组分析揭示了两种不同的PHF19基因启动子在恶性和非恶性肠道粘膜中的活性差异。其中一个启动子在结肠癌中上调，导致PHF19-207转录本的表达，提示其在肿瘤促进中的潜在作用。本研究的目的是利用计算机工具和公开数据研究PHF19-207的功能，并评估其在结肠癌中的表达。通过qPCR和RNA测序对永生化的结肠癌上皮细胞系HCEC-1CT以及一系列在二维和三维环境中培养的结肠癌细胞系中提取的RNA进行表达分析。在两种培养条件下，与非恶性的HCEC-1CT细胞系相比，PHF19-207的表达在所有恶性细胞系中都有所升高，其中在疾病晚期衍生的细胞系和过表达KRAS的HCEC-1CT细胞系中观察到的增加最为显著。此外，在来自恶性细胞的外泌体中检测到PHF19-207转录本。这些发现表明PHF19-207具有作为诊断性生物标志物的潜力。此外，计算机分析表明，该转录物可能作为长链非编码RNA参与基因表达调控。需要进一步的功能研究来阐明其在结肠癌发生中的确切作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.