Enhanced expression and interaction of GmRDR1 and GmSGS3 proteins in resistant soybean cultivars synergistically regulate antiviral defense against mungbean yellow mosaic India virus.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Plant Biology Pub Date : 2025-07-28 DOI:10.1111/plb.70078

D D Chavan, M Sarkar, A Majumdar, F Mondal, Y M Babu, S K Lal, B Mandal, R Kumar, A Roy

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引用次数: 0

Abstract

Mungbean yellow mosaic India virus (MYMIV) causes significant losses to soybean productivity in India. Resistance to MYMIV is reported to be linked with two QTLs. It was hypothesized that within these QTLs, two RNA silencing-related genes, RNA-DEPENDENT RNA POLYMERASE-1 (GmRDR1) and SUPPRESSOR OF GENE SILENCING (GmSGS3), may have a role in governing resistance. In this study, coding regions of the above genes were sequenced from resistant (SL 1074) and susceptible (JS 335) soybean cultivars. While GmRDR1 had identical sequences in both cultivars, two synonymous SNPs in GmSGS3 were identified. Based on one of these SNPs, a CAPS marker was developed, which differentiates resistant and susceptible genotypes. In silico docking and yeast two-hybrid assays confirmed the interaction between GmRDR1 and GmSGS3. Gene expression analysis showed that resistant genotypes expressed higher levels of these transcripts after MYMIV inoculation. Additionally, reducing expression of either gene via RNA interference increased viral accumulation, indicating reduced resistance. This study highlights the critical role of GmRDR1 and GmSGS3 in soybean resistance to MYMIV, suggesting that their enhanced expression and interaction facilitate antiviral defense. Future research should explore molecular pathways involved, which could improve breeding strategies for MYMIV resistance in soybean.

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抗性大豆品种GmRDR1和GmSGS3蛋白的表达和相互作用增强，协同调节对绿豆黄花叶印度病毒的抗病毒防御。

印度绿豆黄花叶病毒（MYMIV）对印度大豆生产力造成重大损失。据报道，对MYMIV的抗性与两个qtl有关。据推测，在这些qtl中，两个RNA沉默相关基因，RNA依赖性RNA聚合酶-1 （GmRDR1）和基因沉默抑制因子（GmSGS3）可能在控制耐药性中起作用。本研究对抗性（SL 1074）和易感（JS 335）大豆品种的上述基因编码区进行了测序。虽然GmRDR1在两个品种中具有相同的序列，但在GmSGS3中发现了两个同义的snp。基于其中一个snp，开发了一个CAPS标记，用于区分耐药和易感基因型。硅对接和酵母双杂交实验证实了GmRDR1和GmSGS3之间的相互作用。基因表达分析显示，在接种MYMIV后，抗性基因型表达了更高水平的这些转录本。此外，通过RNA干扰减少任一基因的表达会增加病毒积累，表明抗性降低。本研究强调了GmRDR1和GmSGS3在大豆抗MYMIV中的关键作用，表明它们的增强表达和相互作用促进了抗病毒防御。未来的研究应进一步探索其中的分子途径，以改进大豆抗MYMIV的育种策略。

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来源期刊

Plant Biology 生物-植物科学

CiteScore

8.20

自引率

2.60%

发文量

109

审稿时长

3 months

期刊介绍： Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.