{"title":"The development of <i>Burkholderia</i> bacteria as heterologous hosts.","authors":"Stephanie C Heard, Alessandra S Eustáquio","doi":"10.1039/d5np00024f","DOIUrl":null,"url":null,"abstract":"<p><p>Covering up to 2024Drug resistance is a serious and growing problem, and new small molecules are needed for a wide variety of clinical and agricultural applications. Natural products, encoded by biosynthetic gene clusters, have consistently been a source of chemical diversity for finely tuned interactions with a range of molecular targets of interest. However, many gene clusters are not transcriptionally active, making heterologous expression in a different host strain a useful tool to access bioactive small molecules. <i>Burkholderia</i> spp. bacteria hold promise as heterologous hosts because of their intrinsic natural product capabilities. In this review, we summarize natural products successfully isolated from <i>Burkholderia</i> spp. heterologous hosts up until 2024. We then compare the hosts that have been tested and discuss ongoing development efforts to improve access to new natural products in titers sufficient for drug development and industrial applications.</p>","PeriodicalId":94,"journal":{"name":"Natural Product Reports","volume":" ","pages":""},"PeriodicalIF":10.6000,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301960/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Reports","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5np00024f","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Covering up to 2024Drug resistance is a serious and growing problem, and new small molecules are needed for a wide variety of clinical and agricultural applications. Natural products, encoded by biosynthetic gene clusters, have consistently been a source of chemical diversity for finely tuned interactions with a range of molecular targets of interest. However, many gene clusters are not transcriptionally active, making heterologous expression in a different host strain a useful tool to access bioactive small molecules. Burkholderia spp. bacteria hold promise as heterologous hosts because of their intrinsic natural product capabilities. In this review, we summarize natural products successfully isolated from Burkholderia spp. heterologous hosts up until 2024. We then compare the hosts that have been tested and discuss ongoing development efforts to improve access to new natural products in titers sufficient for drug development and industrial applications.
期刊介绍：
Natural Product Reports (NPR) serves as a pivotal critical review journal propelling advancements in all facets of natural products research, encompassing isolation, structural and stereochemical determination, biosynthesis, biological activity, and synthesis.
With a broad scope, NPR extends its influence into the wider bioinorganic, bioorganic, and chemical biology communities. Covering areas such as enzymology, nucleic acids, genetics, chemical ecology, carbohydrates, primary and secondary metabolism, and analytical techniques, the journal provides insightful articles focusing on key developments shaping the field, rather than offering exhaustive overviews of all results.
NPR encourages authors to infuse their perspectives on developments, trends, and future directions, fostering a dynamic exchange of ideas within the natural products research community.
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