Entomopathogenic fungal strain Beauveria bassiana GZUIFR-GL5 has potential commercial value for Agrotis ipsilon (Lepidoptera: Noctuidae) control

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-07-28 DOI:10.1016/j.cropro.2025.107348

Ziji Wan , Dongshun Wei , Xiaofei Yu , Xiao Zou , Maofa Yang , Xiang Yang

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引用次数: 0

Abstract

Agrotis ipsilon is a globally significant underground pest. Although Beauveria bassiana exerts a remarkable control effect on A. ipsilon, various strains exhibit different control effects. Therefore, this study treated A. ipsilon larvae with B. bassiana GZUIFR-GL5 (Abbreviation: GL5). The results showed that GL5 could infect A. ipsilon larvae, with virulence gradually increased as the spore concentration increased while gradually decreased as the larval instar stages progressed. Seven days after inoculation, the median lethal concentration (LC₅₀) of GL5 for the second to sixth instar larvae of A. ipsilon was 6.44 × 10⁵, 3.22 × 10⁶, 1.01 × 10⁷, 2.58 × 10⁸, and 1.09 × 10¹⁰ spores/mL, respectively. GL5 also prolonged the developmental cycle of parents as well as reduced the pupation rate, pupal emergence rate, oviposition, larval emergence rate, and egg production; however, no significant effect was determined on the offspring. The activities of protective enzymes (i.e., catalase, peroxidase, and superoxide dismutase) in the second instar significantly changed with infection time and usually lasted from 24 to 48 h after larval infection. Among the different application methods, root irrigation was the best, followed by soil dressing and foliar spraying. Meanwhile, GL5 could achieve the similar control efficacy compared with the commercial strain (Lvhengfeng). The findings of this study suggest that GL5 can be used as a bioinsecticide to control the young larvae of A. ipsilon in integrated pest management programs.

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球孢白僵菌（Beauveria bassiana） GZUIFR-GL5在防治夜蛾方面具有潜在的商业价值

土蛾是一种全球性的地下害虫。虽然球孢白僵菌对白僵菌有显著的防治效果，但不同菌株的防治效果不同。因此，本研究以球孢白僵菌GZUIFR-GL5（简称：GL5）处理伊氏小蠊幼虫。结果表明，GL5可侵染伊氏小蠊幼虫，毒力随孢子浓度的增加而逐渐增强，随幼虫龄期的延长而逐渐减弱。接种7 d后，GL5对2 ~ 6龄小蠹蛾的中位致死浓度（LC50）分别为6.44 × 105、3.22 × 106、1.01 × 107、2.58 × 108和1.09 × 1010孢子/mL。GL5还延长了亲本的发育周期，降低了化蛹率、蛹羽化率、产卵率、幼虫羽化率和产蛋量；然而，对后代没有明显的影响。2龄保护酶（过氧化氢酶、过氧化物酶和超氧化物歧化酶）活性随侵染时间变化显著，通常持续时间为侵染后24 ~ 48 h。不同施用方式中，根灌效果最好，其次是追肥和叶面喷施。同时，GL5的防治效果与商品菌株（绿恒丰）相当。本研究结果表明，GL5可作为一种生物杀虫剂，在害虫综合治理方案中防治伊氏小蠊幼体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.