Simultaneous determination of five active compounds in compound Salvia Miltiorrhiza gel via multi-channel HPLC detection

IF 1.3 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2025-06-10 DOI:10.1016/j.cjac.2025.100577

Hailong Li , Zheng Wang , Qingming Zhang , Wanting Wang , Peipei Han , Haoting Yu , Jiahui Ma , Xingde Zhang , Hui Xie , Hongli Yu

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引用次数: 0

Abstract

Objective

To establish a high-performance liquid chromatographic method (HPLC) for simultaneous determination of two phenolic acids (gallic acid, salvianolic acid B) and three quinones (cryptotanshinone, tanshinone I, tanshinone IIA) in Compound Salvia miltiorrhiza gel (CSG).

Methods

The HPLC method employs gradient elution with multi-channel to optimize detection sensitivity. Validation parameters include linearity, precision, stability, repeatability, and accuracy.

Results

All five compounds exhibit excellent linearity (R² > 0.999) within their respective concentration ranges. The method demonstrates high precision (RSD < 2%), stability (RSD < 1.93%), repeatability (RSD < 1.90%), and accuracy (average recoveries: 98.93–101.31%). No interference is observed in negative control samples.

Conclusion

This validated HPLC method provides a robust and efficient approach for quality control of CSG, ensuring accurate quantification of its key bioactive components. The study supports the standardization of herbal gel formulations and offers a foundation for further pharmacological research.

Abstract Image

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多通道高效液相色谱法同时测定复方丹参凝胶中5种有效成分

目的建立同时测定复方丹参凝胶（CSG）中两种酚酸（没食子酸、丹酚酸B）和三种醌类（隐丹参酮、丹参酮I、丹参酮IIA）含量的高效液相色谱法。方法采用多通道梯度洗脱法优化检测灵敏度。验证参数包括线性度、精密度、稳定性、重复性和准确度。结果5种化合物均表现出良好的线性关系(R2 >；0.999)，均在各自浓度范围内。该方法具有较高的精密度(RSD <；2%)，稳定性(RSD <；1.93%)，重复性(RSD <；平均加样回收率为98.93 ~ 101.31%。在阴性对照样品中未观察到干扰。结论建立的高效液相色谱法可有效控制龙葵药材的质量，保证其主要生物活性成分的准确定量。该研究支持了草药凝胶配方的标准化，并为进一步的药理学研究奠定了基础。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.