Quantitative comparison of thermotolerant Campylobacter spp. growth in Preston broth with and without growth supplement

IF 5.2 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

International journal of food microbiology Pub Date : 2025-07-21 DOI:10.1016/j.ijfoodmicro.2025.111356

Sarah Brueggemann-Schwarze, Sandra Preuß, Juan Cruz Goenaga, Christiane Buhler, Janine Heise, Kerstin Stingl

{"title":"Quantitative comparison of thermotolerant Campylobacter spp. growth in Preston broth with and without growth supplement","authors":"Sarah Brueggemann-Schwarze, Sandra Preuß, Juan Cruz Goenaga, Christiane Buhler, Janine Heise, Kerstin Stingl","doi":"10.1016/j.ijfoodmicro.2025.111356","DOIUrl":null,"url":null,"abstract":"<div><div>Cultural detection of thermotolerant <em>Campylobacter</em> is challenging. ISO 10272-1:2017/Amd1:2023 recommends direct streaking or enrichment with either Bolton broth or Preston broth with growth supplement (FBP).</div><div>From a collection of 123 isolates from food and animal origin, growth in Preston broth without FBP was detected on mCCDA for 56 % <em>C. jejuni</em>, 16 % <em>C. coli</em>, and 10 % <em>C. lari</em> enrichments. Supplementation with FBP improved growth so that all <em>C. jejuni</em>, 67 % <em>C. coli</em> and 85 % <em>C. lari</em> enrichments were positive on mCCDA. In comparison, Bolton broth enrichments were positive for all isolates, except for 7 % <em>C. jejuni</em> enrichments. Different efficiencies of enrichment were confirmed by quantitative Real-time PCR. Enrichment performances of Preston selective supplements from four different companies were compared and FBP concordantly improved <em>Campylobacter</em> detection in Preston broth.</div><div>Moreover, food matrices were inoculated with freshly cultured <em>Campylobacter</em>. The presence of chicken meat, spinach and egg shells but not chicken skin considerably enhanced growth of fresh <em>Campylobacter</em> in Preston. Here, FBP led to no or marginal growth enhancement. Stressed <em>Campylobacter</em> were obtained by adding fresh <em>Campylobacter</em> to food matrices and incubating them aerobically at cold temperature prior to enrichment, mimicking storage conditions. After stress in raw milk, addition of FBP improved the median level of enrichment in Preston by 6.5 log<sub>10</sub> <em>Campylobacter</em>/ml. In other matrices, enrichment levels of stressed cells were slightly higher in Preston with FBP than without. <em>Campylobacter</em> growth in Bolton was highest with 8.8–9.4 log<sub>10</sub> <em>Campylobacter</em>/ml after 48 h.</div><div>In conclusion, FBP improved detection in Preston broth. However, enrichment in Bolton broth was most efficient under our test conditions. Reliable performance evaluation of enrichment media might be monitored by qPCR, using naturally stressed <em>Campylobacter</em> in the presence of food matrix with intrinsic microbiota.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"442 ","pages":"Article 111356"},"PeriodicalIF":5.2000,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168160525003010","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cultural detection of thermotolerant Campylobacter is challenging. ISO 10272-1:2017/Amd1:2023 recommends direct streaking or enrichment with either Bolton broth or Preston broth with growth supplement (FBP).

From a collection of 123 isolates from food and animal origin, growth in Preston broth without FBP was detected on mCCDA for 56 % C. jejuni, 16 % C. coli, and 10 % C. lari enrichments. Supplementation with FBP improved growth so that all C. jejuni, 67 % C. coli and 85 % C. lari enrichments were positive on mCCDA. In comparison, Bolton broth enrichments were positive for all isolates, except for 7 % C. jejuni enrichments. Different efficiencies of enrichment were confirmed by quantitative Real-time PCR. Enrichment performances of Preston selective supplements from four different companies were compared and FBP concordantly improved Campylobacter detection in Preston broth.

Moreover, food matrices were inoculated with freshly cultured Campylobacter. The presence of chicken meat, spinach and egg shells but not chicken skin considerably enhanced growth of fresh Campylobacter in Preston. Here, FBP led to no or marginal growth enhancement. Stressed Campylobacter were obtained by adding fresh Campylobacter to food matrices and incubating them aerobically at cold temperature prior to enrichment, mimicking storage conditions. After stress in raw milk, addition of FBP improved the median level of enrichment in Preston by 6.5 log₁₀ Campylobacter/ml. In other matrices, enrichment levels of stressed cells were slightly higher in Preston with FBP than without. Campylobacter growth in Bolton was highest with 8.8–9.4 log₁₀ Campylobacter/ml after 48 h.

In conclusion, FBP improved detection in Preston broth. However, enrichment in Bolton broth was most efficient under our test conditions. Reliable performance evaluation of enrichment media might be monitored by qPCR, using naturally stressed Campylobacter in the presence of food matrix with intrinsic microbiota.

查看原文本刊更多论文

添加和不添加生长剂对耐热弯曲杆菌在普雷斯顿肉汤中生长的定量比较

耐热弯曲杆菌的培养检测具有挑战性。ISO 10272-1:2017/ amd:2023建议用博尔顿肉汤或普雷斯顿肉汤加生长补剂（FBP）直接划线或富集。从123个来自食品和动物的分离株中，在没有FBP的Preston肉汤中，mcda检测到56%的C.空肠，16%的C.大肠杆菌和10%的C. lari富集。添加FBP促进了细菌的生长，使所有空肠C.、67%的大肠C.和85%的拉里C.在mCCDA上呈阳性。相比之下，除了7%的空肠弧菌外，所有分离株的博尔顿肉汤富集均为阳性。通过实时荧光定量PCR证实了不同的富集效率。比较了4家不同公司的普雷斯顿选择性培养基的富集性能，FBP一致提高了普雷斯顿肉汤中弯曲杆菌的检测。此外，用新鲜培养的弯曲杆菌接种食物基质。鸡肉、菠菜和蛋壳的存在显著促进了新鲜弯曲杆菌在普雷斯顿的生长，但没有鸡皮。在这里，FBP导致没有或边际增长。在食物基质中加入新鲜弯曲杆菌，模拟储存条件，在富集前进行低温好氧培养，获得应激弯曲杆菌。在原料奶中进行应激处理后，添加FBP使Preston中位富集水平提高了6.5 log10 /ml弯曲杆菌。在其他基质中，有FBP的Preston中应激细胞的富集水平略高于没有FBP的Preston。48 h后，Bolton的Campylobacter生长最高，为8.8-9.4 log10 /ml，由此可见，FBP提高了Preston肉汤的检测效果。然而，在我们的测试条件下，在博尔顿肉汤中富集是最有效的。在具有固有微生物群的食物基质存在的情况下，利用自然应激弯曲杆菌进行qPCR可以监测富集培养基的可靠性能评价。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International journal of food microbiology 工程技术-食品科技

CiteScore

10.40

自引率

5.60%

发文量

322

审稿时长

65 days

期刊介绍： The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.