Biology-aware machine learning for culture medium optimization

IF 4.9 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Takamasa Hashizume , Bei-Wen Ying
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引用次数: 0

Abstract

Cell culture technologies are widely used in academia and industry, yet optimizing culture media remains an art due to the complexity of cell-medium interactions. Machine learning has emerged as a promising solution, but it is hindered by biological fluctuations and experimental errors. To address these issues, we developed a medium optimization platform that integrates simplified and effective experimental manipulation, error-aware data processing for model training, predictive model construction to enhance accuracy and avoid local optimization, and an efficient optimization framework of active learning. Using this approach, we fine-tuned a 57-component serum-free medium for CHO-K1 cells, in which a total of 364 media were experimentally tested. The reformulated medium achieved approximately 60 % higher cell concentration than commercial alternatives. The improved cell culture is definitive toward CHO-K1, underscoring the platform's precision in targeted cell culture optimization. Our approach offers a robust tool for optimizing complex systems in cell culture and broader experimental studies, as well as in biomedical engineering applications.
用于培养基优化的生物感知机器学习
细胞培养技术广泛应用于学术界和工业界,但由于细胞-培养基相互作用的复杂性,优化培养基仍然是一门艺术。机器学习已经成为一个很有前途的解决方案,但它受到生物波动和实验误差的阻碍。为了解决这些问题,我们开发了一个中间优化平台,该平台集成了简化有效的实验操作,用于模型训练的错误感知数据处理,用于提高准确性和避免局部优化的预测模型构建,以及一个有效的主动学习优化框架。使用这种方法,我们微调了一种57组分的无血清CHO-K1细胞培养基,其中共有364种培养基进行了实验测试。重新配制的培养基比商业替代品的细胞浓度高约60% %。改进后的细胞培养是针对CHO-K1的,强调了平台在靶向细胞培养优化方面的准确性。我们的方法为优化细胞培养和更广泛的实验研究中的复杂系统以及生物医学工程应用提供了强大的工具。
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来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
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