Dose-dependent X-ray irradiation-induced bystander effects in MCF-7 breast cancer cells.

IF 1.8 3区工程技术 Q3 CHEMISTRY, INORGANIC & NUCLEAR

Applied Radiation and Isotopes Pub Date : 2025-07-22 DOI:10.1016/j.apradiso.2025.112065

Beatriz Allyne D Carandang, Teresa Sy-Ortin, John Paul C Cabahug, Chitho P Feliciano

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引用次数: 0

Abstract

Emerging evidence shows that exposure of cancer cells to ionizing radiation may trigger signal-mediated events in unirradiated cells called the radiation-induced bystander effects (RIBE). Manifestation of the effect is highly variable, dependent on the treatment parameters such as methodology, radiation type, LET, or cell lines. This study aims to investigate RIBE in MCF-7 after irradiation using an X-ray benchtop irradiator. MCF-7 cells were cultured at 2 × 10⁵ cells per T25 flask and irradiated using a CellRad™ bench-top irradiator at 130 kV, 5 mA, with an additional 0.5 mm aluminum filter. The irradiation setup placed the flask within a circular field size of 19.8 cm, delivering a dose rate of 2.096 Gy/min. Acute doses investigated included 0 Gy (sham), 0.5 Gy, 1.5 Gy, 3.36 Gy, 8 Gy, and 12 Gy. Twenty-four hours post-irradiation, medium transfer from irradiated cells (ICM) was performed to initiate radiation-induced bystander effects in non-irradiated cells. Subsequent assessments included viability assays and colony formation assays to validate observed effects, with relative survival calculated relative to sham-irradiated cells. Results show that exposure of the cells to the irradiated cell medium resulted in a significant decrease in cell viability and clonogenic ability, demonstrating bystander effects. This decrease in survival is dose-dependent where the effect is more significant at higher doses 3.36 Gy-12 Gy.

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剂量依赖性x射线照射诱导MCF-7乳腺癌细胞的旁观者效应。

新出现的证据表明，癌细胞暴露于电离辐射可能会在未受辐射的细胞中触发信号介导的事件，称为辐射诱导的旁观者效应（RIBE）。效果的表现是高度可变的，取决于治疗参数，如方法、辐射类型、LET或细胞系。本研究旨在研究x射线台式辐照器辐照后MCF-7的RIBE。每个T25烧瓶培养2 × 105个MCF-7细胞，使用CellRad™台式辐照器在130 kV， 5 mA下辐照，外加0.5 mm铝过滤器。辐照装置将烧瓶置于19.8 cm的圆形场内，剂量率为2.096 Gy/min。研究的急性剂量包括0 Gy（假手术）、0.5 Gy、1.5 Gy、3.36 Gy、8 Gy和12 Gy。辐照24小时后，从辐照细胞（ICM）中转移介质，在未辐照细胞中启动辐射诱导的旁观者效应。随后的评估包括活力测定和菌落形成测定，以验证观察到的效果，并计算相对于假辐照细胞的相对存活率。结果表明，细胞暴露于辐照的细胞培养基中，细胞活力和克隆生成能力显著下降，表现出旁观者效应。这种存活率的降低是剂量依赖性的，在较高剂量（3.36 Gy-12 Gy）时效果更为显著。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Radiation and Isotopes 工程技术-核科学技术

CiteScore

3.00

自引率

12.50%

发文量

406

审稿时长

13.5 months

期刊介绍： Applied Radiation and Isotopes provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and peaceful application of nuclear, radiation and radionuclide techniques in chemistry, physics, biochemistry, biology, medicine, security, engineering and in the earth, planetary and environmental sciences, all including dosimetry. Nuclear techniques are defined in the broadest sense and both experimental and theoretical papers are welcome. They include the development and use of α- and β-particles, X-rays and γ-rays, neutrons and other nuclear particles and radiations from all sources, including radionuclides, synchrotron sources, cyclotrons and reactors and from the natural environment. The journal aims to publish papers with significance to an international audience, containing substantial novelty and scientific impact. The Editors reserve the rights to reject, with or without external review, papers that do not meet these criteria. Papers dealing with radiation processing, i.e., where radiation is used to bring about a biological, chemical or physical change in a material, should be directed to our sister journal Radiation Physics and Chemistry.