A controllable photoresponsive potassium transporter

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-07-28 DOI:10.1038/s41467-025-62113-1

Zhongyan Li, Lin Yuan, Wenju Chang, Junqiu Liu, Jie Shen, Huaqiang Zeng

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Abstract

Artificial photoresponsive transport systems are particularly intriguing because they offer the potential for precise spatio-temporal control, rapid response times and minimal toxicity associated with the light. We report here a controllable, photoresponsive, ion-transporting carrier, achieving controllability through a photoregulated β-cyclodextrin (β-CD)-azobenzene host-guest complex. In this complex, a lipid-anchored β-CD serves as the launch base, tightly immobilizing through host-guest interactions the otherwise freely moving trans-transporter derived from a crown ether-modified trans-azobenzene motif, but rapidly releasing most of it upon 365 nm UV irradiation that converts transporter to its cis-configuration. The launched cis-transporter functions well as a highly efficient potassium transporter, delivering a low EC₅₀ value of 1.51 μM and a drastic transport activity enhancement by ~ 400% relative to the base-bound trans-transporter (EC₅₀ = 7.46 μM), with on-off activities repeatedly regulated by light. In the absence of the base, the on-off activities between cis- and trans-transporters however differ by only 80% (1.35 μM vs 2.45 μM).

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可控光响应钾转运体

人工光响应传输系统特别吸引人，因为它们提供了精确的时空控制、快速的响应时间和最小的与光相关的毒性的潜力。我们在这里报道了一种可控的、光响应的、离子传输载体，通过光调节的β-环糊精(β-CD)-偶氮苯主客体配合物实现了可控性。在该复合物中，脂质锚定的β-CD作为启动基，通过宿主-客体相互作用紧密固定由冠醚修饰的反式偶氮苯基序衍生的自由移动的转运蛋白，但在365 nm紫外线照射下迅速释放大部分转运蛋白，将转运蛋白转化为顺式构型。发射的顺式转运体作为一种高效的钾转运体具有良好的功能，其EC50值较低，仅为1.51 μM，相对于碱基结合转运体（EC50 = 7.46 μM），其转运活性显著增强约400%，且开关活性受光的反复调节。在没有碱基的情况下，顺式转运体和反式转运体之间的开关活性仅相差80% （1.35 μM vs 2.45 μM）。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.