Quantitative lipidomic analysis reveals distinct metabolic traits between stromal cell subpopulations in human orbital adipose tissue

IF 2.7

Metabolism open Pub Date : 2025-07-24 DOI:10.1016/j.metop.2025.100380

Shuwei Tian , Xiaoli Zhang , Jiayong Yu , Juan Cai , Danni Wei , Siqi Li , Pengfei Cai , Wei Song , Suihan Feng , Mengle Shao , Haizhou Li

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Abstract

Adipose tissue, a pivotal metabolic regulator, houses diverse stromal cell populations influencing its dynamic functions. Recent omics studies, including transcriptomics and proteomics, have revealed intricate cellular heterogeneity, yet comprehensive metabolic profiling remains limited. Leveraging fluorescence-activated cell sorting (FACS), we isolated PDGFRα+ DPP4+ and PDGFRα+ DPP4- adipose stromal cells (ASCs) from human orbital adipose tissue (OAT). Integrating gene expression analysis, in vitro adipogenesis assays, and quantitative lipidomics, we characterized their functional and metabolic distinctions. DPP4- ASCs exhibited enhanced adipogenic potential and distinct lipidomic profiles, featuring elevated ceramides and triacylglycerols compared to DPP4+ ASCs. Differential gene expression highlighted metabolic and adipogenic gene signatures reflective of their functional roles in adipose tissue remodeling. Our findings underscore the metabolic heterogeneity within OAT stromal fibroblasts, implicating DPP4- ASCs as potent regulators of adipogenesis and metabolic homeostasis. These insights enhance our understanding of adipose tissue plasticity and may inform therapeutic strategies for conditions like thyroid-associated ophthalmopathy.

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定量脂质组学分析揭示了人眶脂肪组织基质细胞亚群之间不同的代谢特征

脂肪组织是一种关键的代谢调节剂，它容纳了多种影响其动态功能的基质细胞群。最近的组学研究，包括转录组学和蛋白质组学，揭示了复杂的细胞异质性，但全面的代谢分析仍然有限。利用荧光活化细胞分选（FACS），我们从人眼眶脂肪组织（OAT）中分离出PDGFRα+ DPP4+和PDGFRα+ DPP4-脂肪基质细胞（ASCs）。结合基因表达分析、体外脂肪生成测定和定量脂质组学，我们表征了它们的功能和代谢差异。与DPP4+ ASCs相比，DPP4- ASCs表现出增强的成脂潜能和独特的脂质组学特征，其神经酰胺和甘油三酯含量升高。差异基因表达突出了代谢和脂肪生成基因特征，反映了它们在脂肪组织重塑中的功能作用。我们的研究结果强调了OAT间质成纤维细胞的代谢异质性，暗示DPP4- ASCs是脂肪形成和代谢稳态的有效调节因子。这些见解增强了我们对脂肪组织可塑性的理解，并可能为甲状腺相关眼病等疾病的治疗策略提供信息。

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来源期刊

Metabolism open Agricultural and Biological Sciences (General), Endocrinology, Endocrinology, Diabetes and Metabolism

自引率

0.00%

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审稿时长

40 days