Transient overexpression of CpVQ27 reduced the Cucurbita pepo's resistance to powdery mildew

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2025-07-21 DOI:10.1016/j.jplph.2025.154573

Shiheng Li, Ping Wang

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引用次数: 0

Abstract

Powdery mildew (PM) is the main disease in pumpkin (Cucurbita pepo) cultivation and one of the main factors affecting Cucurbita pepo yield. The VQ (Valine-Glutamine) proteins play a crucial role in plant responses to abiotic stresses such as drought and salinity, and biotic stress from pathogenic bacteria. Transcriptomic and qPCR analysis showed that after powdery mildew infection for 24 and 48 h, the FPKM and relative expression of CpVQ27 decreased in disease-resistant material F2 and increased in susceptible material M3. Moreover, the FPKM value and relative expression of CpVQ27 in M3 were higher than those in F2. To investigate CpVQ27's responsiveness to powdery mildew infection, we transiently overexpressed CpVQ27 in virus-susceptible variety MRJ. The GFP fluorescence assay, viral disease phenotyping, and qPCR collectively confirmed the successful transient overexpression of CpVQ27 in Cucurbita pepo. Compared to the control group, transient overexpression of CpVQ27 worsened the powdery mildew disease symptoms. Furthermore, the mycelium of powdery mildew grew faster and accumulated more extensively. There was an increase in ROS and MDA content, along with decreased antioxidant enzyme activities and callose levels. Moreover, the expression levels of defense-related genes were reduced. Transient overexpression of CpVQ27 reduced the Cucurbita pepo's resistance to powdery mildew. Our discoveries lay a solid and comprehensive theoretical foundation for future disease resistance breeding.

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CpVQ27的短暂过表达降低了葫芦对白粉病的抗性

白粉病是南瓜栽培中的主要病害，也是影响南瓜产量的主要因素之一。VQ（缬氨酸-谷氨酰胺）蛋白在植物对干旱和盐度等非生物胁迫以及来自致病菌的生物胁迫的反应中起着至关重要的作用。转录组学和qPCR分析显示，在白粉病感染24和48 h后，CpVQ27的FPKM和相对表达量在抗病材料F2中降低，在感病材料M3中升高。M3的FPKM值和CpVQ27的相对表达量均高于F2。为了研究CpVQ27对白粉病感染的应答性，我们在病毒敏感品种MRJ中短暂过表达CpVQ27。GFP荧光分析、病毒疾病表型分析和qPCR共同证实了CpVQ27在葫芦中成功的过表达。与对照组相比，CpVQ27的短暂过表达加重了白粉病症状。此外，白粉病菌丝体生长速度更快，积累范围更广。活性氧和丙二醛含量升高，抗氧化酶活性和胼胝质含量降低。此外，防御相关基因的表达水平降低。CpVQ27的短暂过表达降低了葫芦对白粉病的抗性。我们的发现为今后的抗病育种奠定了坚实而全面的理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.