Mechanistic insights into the impact of Lincomycin on microalgal-bacterial granular sludge in domestic wastewater treatment

IF 6.3 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-07-25 DOI:10.1016/j.jwpe.2025.108336

Guihua Wang , Shujuan Meng , Bin Ji , Rui Wang , Min Liao , Xinyu Chang , Yu Liu , Meng Zhang

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Abstract

Lincomycin (LCM), a widely used lincosamide antibiotic, poses considerable ecological risks, particularly when residues remain in wastewater. Microalgal-bacterial granular sludge (MBGS) has emerged as a sustainable and efficient technology for domestic wastewater treatment. Nonetheless, the effects of residual LCM on MBGS performance remains largely unexplored. This study examined the impact of varying LCM concentrations on MBGS under simulated domestic wastewater treatment conditions. Our results indicated that 10 mg/L LCM concentrations stimulated MBGS to secrete extracellular polymeric substances (EPS), thereby enhancing both adsorption and biodegradation of LCM. Nevertheless, LCM exposure impaired MBGS efficiency, notably in water treatment and nitrogen cycling processes. Amplicon sequencing revealed that the deterioration of wastewater-treatment efficiency was associated with a reduced abundance of cyanobacteria and bdelloidea. Moreover, metagenomic analysis corroborated that LCM exposure diminished cyanobacteria abundance and highlighted the pivotal role of cyanobacteria in both nitrogen assimilation and dissimilation. LCM exposure impaired nitrogen cycling efficiency, disrupting the production of cofactors production and vitamin metabolism. Furthermore, LCM induced an increase in antibiotic resistance genes (ARGs), which may support MBGS survival under antibiotic stress. This study provides a comprehensive elucidation of the multifaceted impact mechanisms of LCM on MBGS. Additionally, it highlights the critical role of cyanobacteria in nitrogen cycling and their susceptibility to LCM exposure, thereby offering insights into the ecological ramifications of antibiotic pollution in wastewater treatment systems. Overall, this study provides critical insights for the development of more efficient and sustainable MBGS-based wastewater-treatment systems.

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林可霉素对生活污水处理中微藻-细菌颗粒污泥影响的机理研究

林可霉素（LCM）是一种广泛使用的林可沙胺类抗生素，具有相当大的生态风险，特别是当其残留物残留在废水中时。微藻-细菌颗粒污泥（MBGS）已成为一种可持续、高效的生活污水处理技术。尽管如此，残余LCM对MBGS性能的影响在很大程度上仍未被探索。本研究考察了在模拟生活污水处理条件下不同LCM浓度对MBGS的影响。结果表明，浓度为10 mg/L的LCM刺激MBGS分泌胞外聚合物（EPS），从而增强了LCM的吸附和生物降解能力。然而，LCM暴露会损害MBGS的效率，特别是在水处理和氮循环过程中。扩增子测序显示，废水处理效率的恶化与蓝藻和蛭形菌丰度的降低有关。此外，宏基因组分析证实，LCM暴露减少了蓝藻菌的丰度，并强调了蓝藻菌在氮同化和异化中的关键作用。LCM暴露损害了氮循环效率，破坏了辅助因子的产生和维生素代谢。此外，LCM诱导抗生素耐药基因（ARGs）增加，这可能支持MBGS在抗生素胁迫下的存活。本研究全面阐明了LCM对MBGS的多方面影响机制。此外，它强调了蓝藻在氮循环中的关键作用及其对LCM暴露的敏感性，从而提供了对废水处理系统中抗生素污染的生态后果的见解。总的来说，这项研究为开发更有效和可持续的基于mbgs的废水处理系统提供了重要的见解。

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies