The application of desorption electrospray ionization mass spectrometry and mass spectrometry imaging in metabolomics, lipidomics and proteomics analysis

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-07-21 DOI:10.1016/j.talanta.2025.128611

Haofan Liu , Sicheng Huang , Lina Yang , Yaqian He , Yongshuai Jing , Yinghua Xie , Beibei Hu , Zhongqiu Li , Haichao Bi , Zhiwei Li

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Abstract

At present, mass spectrometry (MS) and mass spectrometry imaging (MSI) have developed into versatile analytical techniques, applicable for the analysis of both small molecules and macromolecular compounds and complexes. Desorption electrospray ionization (DESI), as an ambient MS (AMS) technique, enables rapid analysis and imaging under open conditions with minimal or no sample preparation required. In this review, the principles and mechanisms of DESI were described, especially for the factors affecting the analysis including suppression effects, device condition parameter settings, and ionization and transmission efficiency. Moreover, the relevant improvements of DESI technique were introduced in detail. Emphasis has been made to discuss the recent research on the application of DESI MS and MSI in the fields of metabolomics, lipidomics, and proteomics. In these applications, DESI MS and MSI exhibit exceptional capabilities for qualitative analysis.

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解吸电喷雾质谱和质谱成像在代谢组学、脂质组学和蛋白质组学分析中的应用

目前，质谱（MS）和质谱成像（MSI）已经发展成为多功能的分析技术，既适用于小分子分析，也适用于大分子化合物和复合物的分析。解吸电喷雾电离（DESI）作为一种环境质谱（AMS）技术，可以在开放条件下进行快速分析和成像，只需要很少或不需要样品制备。本文综述了DESI的原理和机理，重点介绍了影响DESI分析的因素，包括抑制效果、器件条件参数设置、电离和传输效率。并详细介绍了DESI技术的相关改进。重点讨论了DESI质谱和MSI在代谢组学、脂质组学和蛋白质组学等领域的最新研究进展。在这些应用中，DESI MS和MSI表现出卓越的定性分析能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.