Advanced 3D-Printed hydrogel dressings incorporating platelet-rich plasma for accelerated skin repair.

Abstract

The restoration of cutaneous barrier function following deep skin injury remains a critical challenge in regenerative medicine. In this study, we developed a semi-occlusive wound dressing by combining sodium alginate hydrogel with platelet-rich plasma (PRP), using 3D bioprinting technology to ensure structural precision and consistent bioactive distribution. This hybrid system was engineered to support tissue repair by enhancing re-epithelialization, stimulating angiogenesis, and promoting organized extracellular matrix remodeling. In vivo experiments using full-thickness skin wounds in mice revealed that the PRP enriched dressings accelerated wound contraction and epithelial closure, especially during the early stages of healing. Histological analyses showed increased formation of capillary-like structures, a shift toward type I collagen dominance, and reduced inflammation in PRP treated groups. These effects point to a more mature and functional regenerative process. Importantly, the combination of PRP with a bioprinted hydrogel scaffold not only facilitated structural recovery but also contributed to restoring the physiological integrity of the skin barrier. This approach offers a low-cost and adaptable platform with strong translational potential for the treatment of complex skin wounds.