Evaluating the Cytotoxic, Genotoxic, and Toxic Potential of Pyrolytic Tire Char Using Human Lymphocytes and a Bacterial Biosensor.

IF 3.9 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Toxics Pub Date : 2025-07-12 DOI:10.3390/toxics13070582

Ioanna Efthimiou, Margarita Dormousoglou, Lambrini Giova, Dimitris Vlastos, Stefanos Dailianis, Maria Antonopoulou, Ioannis Konstantinou

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引用次数: 0

Abstract

Waste tires (WTs) constitute a potentially significant source of pollution, and the large quantities that are disposed of require proper handling. Pyrolysis has emerged as an environmentally friendly and effective method for WT treatment. In the present study, the cyto-genotoxic and toxic effects of untreated and acid-treated pyrolytic tire char (PTC_UN and PTC_AT, respectively) were investigated. The cytokinesis block micronucleus (CBMN) assay, using human lymphocytes, and the Aliivibrio fischeri bioluminescence assay were used for the assessment of cyto-genotoxicity and ecotoxicity, respectively. According to the results, both PTC_UN and PTC_AT exhibited genotoxicity at all concentrations tested (2.5, 5, and 10 μg mL^-1), which was more pronounced in the case of PTC_AT. Cytotoxicity induction was reported for PTC_UN and PTC_AT at all concentrations. Both demonstrated a relatively low potential for ecotoxicity induction against A. fischeri. Since the cyto-genotoxic and toxic effects of PTC_AT seemed to be more pronounced, the toxic profile of tire char should be investigated in depth before selecting the appropriate applications, thereby avoiding detrimental effects in the environment and humans alike.

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利用人淋巴细胞和细菌生物传感器评价热解轮胎炭的细胞毒性、基因毒性和毒性潜能。

废轮胎（WTs）是潜在的重要污染源，大量的废弃轮胎需要妥善处理。热解已成为一种环保和有效的WT处理方法。在本研究中，研究了未经处理和酸处理的热解轮胎炭（PTCUN和PTCAT）的细胞遗传毒性和毒性作用。分别采用细胞分裂阻断微核（CBMN）法和费氏弧菌生物发光法评估细胞遗传毒性和生态毒性。结果表明，PTCUN和PTCAT在所有浓度（2.5、5和10 μg mL-1）下均表现出遗传毒性，其中以PTCAT更为明显。所有浓度的PTCUN和PTCAT均有细胞毒性诱导。结果表明，两者对菲氏弧菌的生态毒性诱导潜力较低。由于PTCAT的细胞-基因毒性和毒性作用似乎更为明显，因此在选择适当的应用之前，应深入研究轮胎炭的毒性特征，从而避免对环境和人类产生有害影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxics Chemical Engineering-Chemical Health and Safety

CiteScore

4.50

自引率

10.90%

发文量

681

审稿时长

6 weeks

期刊介绍： Toxics (ISSN 2305-6304) is an international, peer-reviewed, open access journal which provides an advanced forum for studies related to all aspects of toxic chemicals and materials. It publishes reviews, regular research papers, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in detail. There is, therefore, no restriction on the maximum length of the papers, although authors should write their papers in a clear and concise way. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of calculations and experimental procedure can be deposited as supplementary material, if it is not possible to publish them along with the text.