Coupled Effects of Polyethylene Microplastics and Cadmium on Soil-Plant Systems: Impact on Soil Properties and Cadmium Uptake in Lettuce.

Abstract

Microplastics (MPs) and cadmium (Cd) in the soil environment are expected to pose a serious threat to agricultural production. However, the effect of the interaction between them on the soil-plant system and the mechanism of MPs on plant Cd uptake are still unclear. Therefore, the effects of different concentrations of polyethylene (PE-MPs, 0, 1.0% and 2.0%), alone or combined with Cd, on soil properties, plant growth and Cd uptake were investigated through pot experiments. The results showed that the single contamination of MPs and Cd and their interaction (MPs + Cd) significantly decreased soil moisture and pH; however, it increased soil organic matter (SOM) and total nitrogen (TN). Soil urease and catalase activities were significantly decreased and sucrase and alkaline phosphatase activities were increased with or without Cd addition. The exposure of PE and Cd, alone or combined, significantly and negatively affected plant biomass, photosynthetic parameters, and caused oxidative damage to plants, and the overall toxicity to plants increases with the increase in PE concentration. Moreover, co-pollution causes greater plant toxicity than the individual pollution of PE or Cd. Plants can resist oxidative stress by increasing superoxide dismutase (SOD) and peroxidase (POD) activities. The heat map showed that soil environmental factors were significantly correlated with plant growth; and the results of redundancy analysis (RDA) indicated that for plant physiological characteristics, soil properties under PE, alone or co-contaminated with Cd, explained a total of 85.77% and 97.45%, respectively. This indicated that the alteration of the soil microenvironment is the key factor influencing plant growth. The results of the partial least squares path model (PLS-PM) indicated that plant oxidative damage and biomass had significant positive and negative direct effects on plant Cd uptake, respectively. The linear model of relative importance (%) further revealed in depth that soil moisture (relative importance: 33.60%) and plant biomass (relative importance: 20.23%) were, respectively, regarded as the most important soil environmental factors and plant indicators affecting their Cd uptake. This study provided theoretical support for assessing the risks of MPs and Cd co-pollution to agricultural ecosystems.