Jie Wu, Chenchen Zhu, Yin Shu, Yongli Lu, Lu Liu, Zhigang Xiao, Zicheng Li
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引用次数: 0
Abstract
Objective: To examine the effect of Soyasaponin Bb on morphine withdrawal-induced anxiety-like behaviors in male mice and the corresponding mechanism.
Methods: The chronic intermittent escalating-dose morphine administration paradigm and spontaneous withdrawal were used to induce anxiety-like behaviors in male mice. Open field test (OFT) and elevated plus maze test (EPM) were used to evaluate anxiety-like behaviors. Soyasaponin Bb was intragastrically administered. Western blot was used to monitor the level of GSK-3β, Nrf2, and superoxidase dismutase in the basolateral amygdala (BLA). Virus-mediated gene overexpression and knockdown were used to manipulate the expression of GSK-3β and Nrf2 in the BLA. Two-way analysis of variance followed by Tukey's test were used to make comparisons for the experiments between four groups. Student's t-test was used to make comparisons for the experiments between two groups.
Results: Intragastric administration of Soyasaponin Bb attenuated morphine withdrawal-induced anxiety-like behaviors in male mice. Soyasaponin Bb rescued GSK-3β/Nrf2 signaling in the BLA in morphine withdrawn mice. Overexpression of GSK-3β in the BLA was sufficient to induce anxiety-like behaviors in male mice. Knockdown of GSK-3β in the BLA attenuated anxiety-like behaviors in male mice. Knockdown of Nrf2 in the BLA was sufficient to induce anxiety-like behaviors in male mice.
Conclusion: Soyasaponin Bb could play anxiolytic effect by regulating GSK-3β/Nrf2 signaling and inhibition of GSK-3β in the BLA may represent a novel therapeutic approach to morphine addiction and anxiety disorders.
期刊介绍:
The Psychiatry Investigation is published on the 25th day of every month in English by the Korean Neuropsychiatric Association (KNPA). The Journal covers the whole range of psychiatry and neuroscience. Both basic and clinical contributions are encouraged from all disciplines and research areas relevant to the pathophysiology and management of neuropsychiatric disorders and symptoms, as well as researches related to cross cultural psychiatry and ethnic issues in psychiatry. The Journal publishes editorials, review articles, original articles, brief reports, viewpoints and correspondences. All research articles are peer reviewed. Contributions are accepted for publication on the condition that their substance has not been published or submitted for publication elsewhere. Authors submitting papers to the Journal (serially or otherwise) with a common theme or using data derived from the same sample (or a subset thereof) must send details of all relevant previous publications and simultaneous submissions. The Journal is not responsible for statements made by contributors. Material in the Journal does not necessarily reflect the views of the Editor or of the KNPA. Manuscripts accepted for publication are copy-edited to improve readability and to ensure conformity with house style.