Soyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling.

IF 1.8 4区 医学 Q3 PSYCHIATRY
Psychiatry Investigation Pub Date : 2025-07-01 Epub Date: 2025-07-16 DOI:10.30773/pi.2025.0091
Jie Wu, Chenchen Zhu, Yin Shu, Yongli Lu, Lu Liu, Zhigang Xiao, Zicheng Li
{"title":"Soyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling.","authors":"Jie Wu, Chenchen Zhu, Yin Shu, Yongli Lu, Lu Liu, Zhigang Xiao, Zicheng Li","doi":"10.30773/pi.2025.0091","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To examine the effect of Soyasaponin Bb on morphine withdrawal-induced anxiety-like behaviors in male mice and the corresponding mechanism.</p><p><strong>Methods: </strong>The chronic intermittent escalating-dose morphine administration paradigm and spontaneous withdrawal were used to induce anxiety-like behaviors in male mice. Open field test (OFT) and elevated plus maze test (EPM) were used to evaluate anxiety-like behaviors. Soyasaponin Bb was intragastrically administered. Western blot was used to monitor the level of GSK-3β, Nrf2, and superoxidase dismutase in the basolateral amygdala (BLA). Virus-mediated gene overexpression and knockdown were used to manipulate the expression of GSK-3β and Nrf2 in the BLA. Two-way analysis of variance followed by Tukey's test were used to make comparisons for the experiments between four groups. Student's t-test was used to make comparisons for the experiments between two groups.</p><p><strong>Results: </strong>Intragastric administration of Soyasaponin Bb attenuated morphine withdrawal-induced anxiety-like behaviors in male mice. Soyasaponin Bb rescued GSK-3β/Nrf2 signaling in the BLA in morphine withdrawn mice. Overexpression of GSK-3β in the BLA was sufficient to induce anxiety-like behaviors in male mice. Knockdown of GSK-3β in the BLA attenuated anxiety-like behaviors in male mice. Knockdown of Nrf2 in the BLA was sufficient to induce anxiety-like behaviors in male mice.</p><p><strong>Conclusion: </strong>Soyasaponin Bb could play anxiolytic effect by regulating GSK-3β/Nrf2 signaling and inhibition of GSK-3β in the BLA may represent a novel therapeutic approach to morphine addiction and anxiety disorders.</p>","PeriodicalId":21164,"journal":{"name":"Psychiatry Investigation","volume":"22 7","pages":"821-831"},"PeriodicalIF":1.8000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301682/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Psychiatry Investigation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.30773/pi.2025.0091","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/16 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PSYCHIATRY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: To examine the effect of Soyasaponin Bb on morphine withdrawal-induced anxiety-like behaviors in male mice and the corresponding mechanism.

Methods: The chronic intermittent escalating-dose morphine administration paradigm and spontaneous withdrawal were used to induce anxiety-like behaviors in male mice. Open field test (OFT) and elevated plus maze test (EPM) were used to evaluate anxiety-like behaviors. Soyasaponin Bb was intragastrically administered. Western blot was used to monitor the level of GSK-3β, Nrf2, and superoxidase dismutase in the basolateral amygdala (BLA). Virus-mediated gene overexpression and knockdown were used to manipulate the expression of GSK-3β and Nrf2 in the BLA. Two-way analysis of variance followed by Tukey's test were used to make comparisons for the experiments between four groups. Student's t-test was used to make comparisons for the experiments between two groups.

Results: Intragastric administration of Soyasaponin Bb attenuated morphine withdrawal-induced anxiety-like behaviors in male mice. Soyasaponin Bb rescued GSK-3β/Nrf2 signaling in the BLA in morphine withdrawn mice. Overexpression of GSK-3β in the BLA was sufficient to induce anxiety-like behaviors in male mice. Knockdown of GSK-3β in the BLA attenuated anxiety-like behaviors in male mice. Knockdown of Nrf2 in the BLA was sufficient to induce anxiety-like behaviors in male mice.

Conclusion: Soyasaponin Bb could play anxiolytic effect by regulating GSK-3β/Nrf2 signaling and inhibition of GSK-3β in the BLA may represent a novel therapeutic approach to morphine addiction and anxiety disorders.

Abstract Image

Abstract Image

Abstract Image

大豆皂苷Bb通过挽救GSK-3β/Nrf2信号通路减轻吗啡戒断诱导的雄性小鼠焦虑样行为。
目的:探讨大豆皂苷Bb对吗啡戒断诱导的雄性小鼠焦虑样行为的影响及其机制。方法:采用慢性间歇性递增剂量吗啡给药模式和自发戒断模式诱导雄性小鼠焦虑样行为。采用开阔场测验(OFT)和升高迷宫测验(EPM)评价焦虑样行为。大豆皂苷Bb灌胃。Western blot检测大鼠基底外侧杏仁核(BLA)中GSK-3β、Nrf2、超氧化酶歧化酶水平。利用病毒介导的基因过表达和敲低调控GSK-3β和Nrf2在BLA中的表达。采用双向方差分析和Tukey检验对四组实验进行比较。采用学生t检验对两组实验进行比较。结果:大鼠灌胃大豆皂苷Bb可减轻吗啡戒断引起的焦虑样行为。大豆皂苷Bb可改善吗啡戒断小鼠BLA中GSK-3β/Nrf2信号通路。BLA中GSK-3β的过表达足以诱导雄性小鼠的焦虑样行为。敲低BLA中的GSK-3β可减轻雄性小鼠的焦虑样行为。在雄性小鼠中,敲低BLA中的Nrf2足以诱导焦虑样行为。结论:大豆皂苷Bb可能通过调节GSK-3β/Nrf2信号通路发挥抗焦虑作用,抑制GSK-3β在BLA中的表达可能是治疗吗啡成瘾和焦虑障碍的新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.10
自引率
3.70%
发文量
105
审稿时长
6-12 weeks
期刊介绍: The Psychiatry Investigation is published on the 25th day of every month in English by the Korean Neuropsychiatric Association (KNPA). The Journal covers the whole range of psychiatry and neuroscience. Both basic and clinical contributions are encouraged from all disciplines and research areas relevant to the pathophysiology and management of neuropsychiatric disorders and symptoms, as well as researches related to cross cultural psychiatry and ethnic issues in psychiatry. The Journal publishes editorials, review articles, original articles, brief reports, viewpoints and correspondences. All research articles are peer reviewed. Contributions are accepted for publication on the condition that their substance has not been published or submitted for publication elsewhere. Authors submitting papers to the Journal (serially or otherwise) with a common theme or using data derived from the same sample (or a subset thereof) must send details of all relevant previous publications and simultaneous submissions. The Journal is not responsible for statements made by contributors. Material in the Journal does not necessarily reflect the views of the Editor or of the KNPA. Manuscripts accepted for publication are copy-edited to improve readability and to ensure conformity with house style.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信