Multi-Omics Analysis of Gut Microbiota and Sperm Quality in Tibetan Breeding Boars.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-07-02 DOI:10.3390/metabo15070447

Mingxuan Zhao, Mengjia Han, Hongliang Zhang, Xiangdong Wang, Yikai Yin, Jian Zhang, Peng Shang

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引用次数: 0

Abstract

Background/objectives: Reproductive efficiency in breeding boars critically impacts swine industry productivity, with sperm quality being multifactorially regulated by gut microbiota. This study aimed to elucidate the microbiota-metabolite interactions underlying sperm quality differences in Tibetan boars.

Methods: Integrated 16S rRNA sequencing and untargeted metabolomics were performed on fecal and semen samples from eight healthy Tibetan boars (31-33 months old), stratified into low-semen (CJ) and high-semen utilization (HJ) groups. Analyses included sperm quality assessment, microbial profiling, and metabolic pathway enrichment.

Results: The HJ group exhibited significantly enhanced sperm motility and semen utilization rates (p < 0.05). Gut microbiota composition differed markedly, with Firmicutes and Proteobacteria enriched in HJ boars. Metabolomics identified key metabolites positively correlated with sperm quality (e.g., butyrate, phenyllactic acid), while lithocholic acid showed negative associations. KEGG analysis revealed predominant involvement in butanoate metabolism and bile acid biosynthesis. Core microbiota (e.g., Ruminococcus) modulated sperm quality through short-chain fatty acid networks and bile acid homeostasis.

Conclusions: Gut microbiota regulated the sperm microenvironment via a "metabolic-immune" dual pathway mediated by the gut-testis axis. These findings establish a theoretical basis for probiotic or metabolite-targeted strategies to improve boar reproductive performance.

查看原文本刊更多论文

藏种公猪肠道微生物群与精子质量的多组学分析。

背景/目的：由于精子质量受肠道微生物群的多因素调节，种猪的繁殖效率对养猪业的生产力有重要影响。本研究旨在阐明藏公猪精子质量差异背后的微生物群-代谢物相互作用。方法：对8头31 ~ 33月龄健康藏公猪粪便和精液样本进行16S rRNA测序和非靶向代谢组学分析，将其分为低精液（CJ）组和高精液利用（HJ）组。分析包括精子质量评估、微生物谱分析和代谢途径富集。结果：HJ组精子活力和精液利用率显著提高（p < 0.05）。肠道菌群组成差异显著，厚壁菌门和变形菌门在HJ公猪中富集。代谢组学鉴定出与精子质量呈正相关的关键代谢物（如丁酸盐、苯乳酸），而石胆酸呈负相关。KEGG分析显示主要参与丁酸代谢和胆汁酸生物合成。核心微生物群（如瘤胃球菌）通过短链脂肪酸网络和胆汁酸稳态调节精子质量。结论：肠道微生物群通过肠道-睾丸轴介导的“代谢-免疫”双途径调节精子微环境。这些发现为益生菌或代谢物靶向策略提高公猪繁殖性能奠定了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.