Serum Circ-DLGAP4 and miR-9 as Potential Biomarkers in Pancreatic Cancer

IF 2.9 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-07-25 DOI:10.1002/jcla.70076

Marwa A. Ali, Olfat G. Shaker, Eman M. Ezzat, Mohamed Saad Zaghloul Ahmed, Shimaa Mohammed Elasmer, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Zaidan, Raghda Yahia Abu El-Ela, Marwa N. AbdelHafez, Eman Fares, Ahmad A. Al Abdulqader, Aeshah A. Awaji, Mariam K. Alamoudi, Omayma O. Abdelaleem

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引用次数: 0

Abstract

Background

Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA-DLGAP4 (Circ-DLGAP4) and microRNA-9 (miR-9) are intriguing, easily accessible genetic indicators for numerous diseases.

Aim

To quantify serum expression levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.

Method

Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT-PCR.

Results

There were statistically significant elevations in blood levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39–17.32), p-value < 0.001, and 9.5 (4.06–14.7), respectively. The blood level of Circ-DLGAP4 exhibited a significant positive correlation with the serum concentration of miR-9 (r = 0.89, p = 0.001).

Conclusions

Circ-DLGAP4 and miR-9 serve as diagnostic biomarkers for pancreatic cancer.

Abstract Image

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血清Circ-DLGAP4和miR-9作为胰腺癌的潜在生物标志物

背景：胰腺癌是发病最隐匿、预后最恶劣的恶性肿瘤之一。初步诊断的生物标志物对于及时识别和改善预后至关重要。circularna - dlgap4 （Circ-DLGAP4）和microRNA-9 （miR-9）是许多疾病的有趣的、容易获得的遗传指标。目的：量化胰腺癌患者血清中Circ-DLGAP4和miR-9的表达水平，并将这些值与对照组进行比较，以评估其作为诊断性生物标志物的潜力。它还旨在将它们的表达水平与疾病的临床病理特征联系起来。方法：采用RT-PCR方法对40例胰腺癌患者和40例对照者的血清进行定量分析。结果：胰腺癌患者血液中Circ-DLGAP4和miR-9水平与健康人相比有统计学意义的升高，中位（范围）值为8.2 (0.39-17.32)，p值结论：Circ-DLGAP4和miR-9可作为胰腺癌的诊断生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.