Label-Free Sorting of Human Mesenchymal Stem Cells Using Insulating Dielectrophoresis.

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-07-24 DOI:10.1002/elps.70001

Zuri A Rashad, Kiara L Lacy, Emmanuel Egun, Jazmine S Moore, Tayloria N G Adams

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引用次数: 0

Abstract

Human mesenchymal stem cells (hMSCs) are a multipotent yet heterogeneous cell population with immunosuppressive and regenerative properties, making them highly promising for stem cell therapies targeting metabolic diseases. However, the inherent heterogeneity of hMSCs presents challenges for producing consistent therapeutic outcomes, emphasizing the need to isolate functionally distinct subpopulations. In this study, we employed insulating dielectrophoresis (DEP) via a trap-and-release sorting strategy to generate and characterize subpopulations of adipose tissue (AT)-derived hMSCs. Voltage and frequency parameters were systematically tuned, revealing that higher voltages increased the percentage of trapped cells, while higher frequencies had less impact. Sorted cells underwent a 14-day adipogenic differentiation process, assessed by Oil Red O staining. Our results demonstrated that untrapped cell populations generated at lower voltage and frequency thresholds exhibited enhanced adipogenic differentiation compared to unsorted controls. These findings suggest that DEP can be leveraged to isolate progenitor cells within hMSC populations, enabling the production of homogeneous cell subsets with targeted functional potential. This work highlights the utility of insulating DEP for addressing hMSC heterogeneity and advancing the development of stem cell therapies.

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人间充质干细胞绝缘介质电泳的无标记分选。

人间充质干细胞（hMSCs）是一种多能但异质性的细胞群，具有免疫抑制和再生特性，使其在针对代谢性疾病的干细胞治疗中具有很大的前景。然而，hMSCs固有的异质性为产生一致的治疗结果提出了挑战，强调需要分离功能不同的亚群。在这项研究中，我们采用绝缘电介质电泳（DEP）通过捕获-释放分选策略来生成和表征脂肪组织（AT）来源的hMSCs亚群。系统地调整了电压和频率参数，发现较高的电压增加了捕获细胞的百分比，而较高的频率影响较小。分类后的细胞经过14天的成脂分化过程，通过油红O染色评估。我们的研究结果表明，与未分类的对照相比，在较低电压和频率阈值下产生的未捕获细胞群表现出更强的成脂分化。这些发现表明，DEP可以用于分离hMSC群体中的祖细胞，从而产生具有目标功能潜力的均匀细胞亚群。这项工作强调了绝缘DEP在解决hMSC异质性和推进干细胞治疗发展方面的效用。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.