New way to isolate cultured cell exosomes.

Abstract

Exosomes are one of the extracellular vesicles that are secreted by almost all cell types and body fluids. Because they are nanosized (30-200 nm), they can be used as natural nanovesicles. Exosomes have recently been preferred for their low immunogenicity and toxicity features for cell-free therapy, nano-drug carriers, and regenerative medicine. Rapid and appropriate exosome isolation has become increasingly critical due to its extensive application area. In this study, we isolated the MCF-7 cell exosomes using a biological membrane that works for nanoparticle isolation. Our results showed that the number of exosomes was 2 × 106 particles per ml in the cell line media, with a peak size of 110 nm. The proposed technique has features such as simplifying the operative procedures, low cost, and high efficiency. In addition, this technique did not use high-cost reactants, and it was not time-consuming. Additionally, no further procedure was necessary, and the amount of hand manipulation was minimal.