Exploration of the mechanism underlying the impact of artificial light on guinea pig ovarian function via proteomics and acetylation analyses.

IF 6.2 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2025-07-23 DOI:10.1016/j.ecoenv.2025.118719

Jiayu Shi, Yuliang Wang, Linfang Li, Ruxin Wang, Yao Peng, Tingting Zhou, Donglai Hu, Jie Chen, Yong Tan

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引用次数: 0

Abstract

Objective: This study aims to explore the effects of artificial light on ovarian function in female guinea pigs and the possible mechanism.

Methods: The levels of serum sex hormone expression in the natural light group (N) and LED group (L) were detected via ELISA. The differentially expressed proteins and changes to acetylation levels in the ovaries of female guinea pigs from both groups were analysed via 4D-DIA proteomics and 4D-DIA acetylation quantitative proteomics. The potential mechanisms by which artificial light causes ovarian dysfunction in female guinea pigs were identified, and the results of proteomics and acetylation modification studies were validated via WB and IHC.

Results: The androgen, oestrogen, dehydroepiandrosterone sulphate, and luteinizing hormone levels in guinea pig serum, were lower in the LED group than in the natural light group. Follicle-stimulating hormone and sex hormone-binding globulin exhibit the opposite trend. Proteomics showed the decrease of protein expression levels of StAR, which was verified by WB and IHC. Quantitative proteomics for acetylation revealed that Bmal1 underwent acetylation at 111 sites. The level of Bmal1 acetylation in guinea pig ovaries was verified via WB, which revealed an increasing trend. WB and IHC revealed an increase in the protein levels of TIP60 and Cry1.

Conclusion: Artificial light affects ovarian dysfunction in female guinea pigs may involve the acetylation at site 111 of Bmal1. This affects Cry1 accumulation, inhibits the transcriptional activity of the circadian gene loop, influences StAR formation, and consequently impacts their ovarian function.

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通过蛋白质组学和乙酰化分析探索人造光对豚鼠卵巢功能影响的机制。

目的：探讨人工光对雌性豚鼠卵巢功能的影响及其可能的机制。方法：采用ELISA法检测自然光组(N)和LED组(L)大鼠血清性激素表达水平。通过4D-DIA蛋白质组学和4D-DIA乙酰化定量蛋白质组学分析两组雌性豚鼠卵巢中差异表达蛋白和乙酰化水平的变化。研究了人造光引起雌性豚鼠卵巢功能障碍的潜在机制，并通过WB和IHC验证了蛋白质组学和乙酰化修饰研究的结果。结果：LED组豚鼠血清中雄激素、雌激素、硫酸脱氢表雄酮和黄体生成素水平均低于自然光组。促卵泡激素和性激素结合球蛋白表现出相反的趋势。蛋白质组学显示StAR蛋白表达水平下降，WB和IHC证实了这一点。乙酰化的定量蛋白质组学显示Bmal1在111个位点发生了乙酰化。WB证实豚鼠卵巢中Bmal1乙酰化水平呈上升趋势。WB和IHC显示TIP60和Cry1蛋白水平升高。结论：人工光照对雌性豚鼠卵巢功能障碍的影响可能与Bmal1 111位点乙酰化有关。这影响了Cry1的积累，抑制了昼夜节律基因环的转录活性，影响了StAR的形成，从而影响了卵巢功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.