{"title":"A Golgi-targeting fluorescent probe for the detection of hydrazine in biological and environmental systems.","authors":"Hui-Juan Lai, Xu Wang, Yuan Wang, Wei-Na Wu, Zhi-Hong Xu","doi":"10.1039/d5ay00690b","DOIUrl":null,"url":null,"abstract":"<p><p>Hydrazine (N<sub>2</sub>H<sub>4</sub>) is regarded as an extremely toxic agent but it is used in large quantities as a chemical raw material in various industries, which poses a severe danger to both the environment and public health. Notably, the Golgi apparatus (GA) of hepatocytes is frequently affected by toxins in cases of hepatotoxicity. Therefore, developing a probe to monitor N<sub>2</sub>H<sub>4</sub> in environmental systems and within the GA of live cells is highly valuable. A Golgi-targeting fluorescent probe, BHMBS, is synthesized to detect N<sub>2</sub>H<sub>4</sub>. The probe BHMBS emits faint green fluorescence at 550 nm, which is remarkably enhanced after adding N<sub>2</sub>H<sub>4</sub>. The probe can detect N<sub>2</sub>H<sub>4</sub> with an ultra-short reaction time, high sensitivity, and excellent selectivity, making it a valuable tool to monitor N<sub>2</sub>H<sub>4</sub> in the real world and image the N<sub>2</sub>H<sub>4</sub> fluctuations in cells and zebrafish.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5ay00690b","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Hydrazine (N2H4) is regarded as an extremely toxic agent but it is used in large quantities as a chemical raw material in various industries, which poses a severe danger to both the environment and public health. Notably, the Golgi apparatus (GA) of hepatocytes is frequently affected by toxins in cases of hepatotoxicity. Therefore, developing a probe to monitor N2H4 in environmental systems and within the GA of live cells is highly valuable. A Golgi-targeting fluorescent probe, BHMBS, is synthesized to detect N2H4. The probe BHMBS emits faint green fluorescence at 550 nm, which is remarkably enhanced after adding N2H4. The probe can detect N2H4 with an ultra-short reaction time, high sensitivity, and excellent selectivity, making it a valuable tool to monitor N2H4 in the real world and image the N2H4 fluctuations in cells and zebrafish.
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