Thermodynamics and Single-Site Interactions between Human Serum Albumin and Green Low-Melting Mixture Solvents.

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-07-25 DOI:10.1021/acs.biomac.5c01213

Li Fu, Xiaoxia Peng, Yaxue Shen, Zhuojia Shi, Ziying Zhen, Ran Sun, Yaqi Wu, Liu Lu, Yu Chen

引用次数: 0

Abstract

Thermodynamics and interaction between human serum albumin (HSA) and low-melting mixture solvents (LoMMSs) play a critical role in the green and low-cost separation of proteins. To the best of our knowledge, there have been no reports on this issue to date. Here, we present the thermodynamic properties and single-site interaction between biobased LoMMSs betaine:glycerol and HSA. LoMMSs demonstrate strong binding affinity toward HSA, driven primarily by van der Waals forces and hydrogen bonding. LoMMSs can stabilize the secondary structure of HSA, preserving its functional integrity. This work provides valuable insights to design green, sustainable, and cost-effective LoMMSs for protein purification.

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人血清白蛋白与绿色低熔点混合溶剂的热力学和单位点相互作用。

人血清白蛋白（HSA）与低熔点混合溶剂（LoMMSs）的热力学和相互作用在绿色低成本的蛋白质分离中起着至关重要的作用。据我们所知，到目前为止还没有关于这个问题的报道。在这里，我们介绍了生物基LoMMSs甜菜碱：甘油和HSA之间的热力学性质和单位点相互作用。LoMMSs对HSA具有很强的结合亲和力，主要由范德华力和氢键驱动。LoMMSs可以稳定HSA的二级结构，保持其功能完整性。这项工作为设计绿色、可持续和具有成本效益的蛋白质纯化LoMMSs提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.