Development of a hemicyanine-based turn-on probe for MAO-A imaging in metabolic disorders in vivo and in serum

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-07-24 DOI:10.1016/j.saa.2025.126736

Lu Fan , Yulong Jin , Aiguo Zhang , Youzhen Feng , Kai Wang

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Abstract

In this study, A fluorescent/photoacoustic dual-modal probe HD-MAO for hemicyanine derivatives was successfully designed and synthesized, achieving high-sensitivity and high-specificity detection of monoamine oxidase A (MAO-A). Through the ingenious combination of the specific MAO-A recognition group and the hemipanocyanine fluorophore, the probe produced a significant enhancement of the intramolecular charge transfer (ICT) effect after enzymatic digestion, showing the synergistic activation characteristics of fluorescence (∼730 nm) and photoacoustic (∼700 nm) signals, with a detection limit of 0.806 mg/L. In the diet-induced obese mouse model, HD-MAO successfully revealed a significant increase in liver MAO-A activity, which was highly consistent with the serum test results, providing molecular imaging evidence for the early diagnosis of metabolic diseases. The probe behaves stably under physiological conditions and shows no cross-reaction to common interferents. Serum detection experiments further confirm its clinical applicability. This study not only provides an innovative tool for the research on MAO-A-related mechanisms, but also opens up a new approach for the early diagnosis and treatment monitoring of metabolic diseases.

Abstract Image

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基于半花青碱的MAO-A在体内和血清代谢紊乱成像的开启探针的开发

本研究成功设计并合成了半花青碱类衍生物荧光/光声双峰探针HD-MAO，实现了对单胺氧化酶A （MAO-A）的高灵敏度和高特异性检测。通过特异性MAO-A识别基团与半花青素荧光团的巧妙结合，该探针在酶解后产生了显著增强的分子内电荷转移（ICT）效应，表现出荧光（~ 730 nm）和光声（~ 700 nm）信号的协同激活特性，检出限为0.806 mg/L。在饮食性肥胖小鼠模型中，HD-MAO成功显示肝脏MAO-A活性显著升高，这与血清检测结果高度一致，为代谢性疾病的早期诊断提供了分子影像学证据。该探针在生理条件下性能稳定，对常见干扰无交叉反应。血清检测实验进一步证实了其临床适用性。本研究不仅为研究mao - a相关机制提供了创新工具，也为代谢性疾病的早期诊断和治疗监测开辟了新途径。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.