Bio-fabricated CuO nanoparticles using Rhinacanthus nasutus leaf extract for antimicrobial and photocatalytic facets

M. Aswin , Prammitha Rajaram , Ambrose Rejo Jeice
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Abstract

The increasing contamination of water sources poses a serious threat to clean water availability. Photocatalytic degradation offers a sustainable solution for wastewater treatment by utilizing light energy to break down pollutants without generating secondary waste. In this study, Rhinacanthus nasutus leaf extract serves as a green reducing and stabilizing agent for the eco-friendly synthesis of CuO bio-nanoparticles (BNPs). The bioactive compounds in the extract facilitate NPs formation while enhancing stability and functional properties. Various characterization techniques, including XRD, UV-Vis spectroscopy, FTIR, SEM-EDX, micro-Raman, and HRTEM analysis, were employed. The bio-fabricated CuO BNPs as monoclinic in crystalline structure with 19 nm size, and 1.61 eV band gap energy of CuO BNPs via UV–vis spectra and irregular spherical shape in morphology were found. The findings of the antimicrobial investigation demonstrated that the CuO BNPs caused the zones that were inhibitors to microorganisms as S. aureus, E. coli and A. flavus. Against three cationic dyes, Methylene Blue, Brilliant Green, and Rhodamine B, CuO NPs demonstrated photocatalytic activity, reaching degradation efficiencies of 92.86 %, 92.83 %, and 93.44 %, respectively.
利用鼻棘叶提取物制备纳米CuO用于抗菌和光催化方面
水源的污染日益严重,对清洁用水的供应构成严重威胁。光催化降解通过利用光能分解污染物而不产生二次废物,为废水处理提供了一种可持续的解决方案。在本研究中,牛角犀叶提取物作为绿色还原剂和稳定剂用于生态合成CuO生物纳米颗粒(BNPs)。提取物中的生物活性化合物促进NPs的形成,同时增强稳定性和功能特性。采用了各种表征技术,包括XRD, UV-Vis光谱,FTIR, SEM-EDX, micro-Raman和HRTEM分析。生物合成的CuO BNPs晶体结构为单斜晶,尺寸为19 nm,紫外可见光谱显示CuO BNPs带隙能为1.61 eV,形貌呈不规则球形。抗菌研究结果表明,CuO BNPs产生了抑制金黄色葡萄球菌、大肠杆菌和黄芽孢杆菌等微生物的区域。对于三种阳离子染料,亚甲基蓝,亮绿和罗丹明B, CuO NPs表现出光催化活性,降解效率分别达到92.86 %,92.83 %和93.44 %。
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