Deciphering the response of pyrite/sulfur autotrophic denitrification system to sulfamethoxazole and copper stress: Insights from microbial community and antibiotic resistance genes

IF 6.3 2区 工程技术 Q1 ENGINEERING, CHEMICAL
Zhenyu Wang , Xuejiang Wang , Zaoli Gu , Ben Dai , Jianzhuo Zhou , Dianhai Yang , Siqing Xia
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引用次数: 0

Abstract

Antibiotics disrupt microbial community in biological treatment systems and promote development of antibiotic resistance genes (ARGs), with heavy metals amplifying these risks. This study developed three autotrophic denitrification systems driven by solid electron donors (pyrite, Rp; elemental sulfur, Rs; mixture of pyrite and elemental sulfur, Rm) to investigate the effects of sulfamethoxazole (SMX) and the combined stress of Cu(II). Results indicated that 0.2–5 mg/L SMX minimally affected the nitrogen removal efficiency of Rm (99.18 %–99.66 %), but 5 mg/L SMX and 1 mg/L Cu(II) co-stress reduced it to 93.82 %, while Rs maintained excellent performance (99.25 %–99.27 %) and Rp consistently underperformed (20.67 %–22.39 %). Excitation-emission matrix-parallel factor analysis suggested that Cu(II) inhibited metabolic activity, reflected by decreased soluble microbial product contents. Stochastic processes mainly governed community assembly, with drift driving the shift from Thiobacillus to Ferritrophicum in Rs and Rm, while diffusion limitation dispersed bacterial composition in Rp. Rm exhibited the most complex ecological network dominated by microbial cooperation. Despite the downregulation of denitrification and sulfur oxidation genes under stress, upregulated ABC transport and electron transport genes (especially in Rs/Rm) likely ensured stable nitrogen removal. Additionally, SMX persistently enriched ARGs, exacerbated by Cu(II), yet surprisingly, pyrite proportion negatively correlated with ARGs abundance. Compared to Rs, Rm significantly lessened the potential ARGs hosts, while Rp showed no multi-ARG hosts. This study offers valuable insights into the microbial community response and ARGs spread in solid-phase autotrophic denitrification systems under antibiotic and heavy metal stress.

Abstract Image

解读黄铁矿/硫自养反硝化系统对磺胺甲恶唑和铜胁迫的响应:来自微生物群落和抗生素耐药基因的见解
抗生素破坏生物处理系统中的微生物群落并促进抗生素耐药基因(ARGs)的发展,而重金属则放大了这些风险。本研究开发了三种由固体电子供体(黄铁矿、Rp;单质硫Rs;研究磺胺甲恶唑(SMX)和Cu(II)复合应力的影响。结果表明,0.2 ~ 5 mg/L SMX对Rm的脱氮效率影响最小(99.18% ~ 99.66%),而5 mg/L SMX和1 mg/L Cu(II)共胁迫使Rm的脱氮效率降低至93.82%,Rs的脱氮效率保持良好(99.25% ~ 99.27%),Rp的脱氮效率一直较差(20.67% ~ 22.39%)。激发-发射矩阵-平行因子分析表明,Cu(II)抑制了代谢活性,表现为可溶性微生物产物含量的降低。随机过程主要控制群落的聚集,漂移驱动Rs和Rm中硫杆菌向嗜铁杆菌的转变,而扩散限制分散了Rp中的细菌组成。Rm表现出以微生物合作为主的最复杂的生态网络。尽管反硝化和硫氧化基因在胁迫下下调,但ABC转运和电子转运基因(尤其是Rs/Rm)的上调可能确保了氮的稳定脱除。此外,SMX持续富集ARGs, Cu(II)加剧了ARGs的富集,但令人惊讶的是,黄铁矿比例与ARGs丰度呈负相关。与Rs相比,Rm明显减少了潜在arg宿主,而Rp没有显示多arg宿主。本研究为抗生素和重金属胁迫下固相自养反硝化系统中微生物群落的反应和ARGs的传播提供了有价值的见解。
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来源期刊
Journal of water process engineering
Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
10.70
自引率
8.60%
发文量
846
审稿时长
24 days
期刊介绍: The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies
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