An enzyme for high-yield, ATP-driven C-terminal thioester generation

IF 19.2 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nature chemistry Pub Date : 2025-07-25 DOI:10.1038/s41557-025-01872-2

引用次数: 0

Abstract

Current tools for protein bioconjugation at the C-terminal have limited yields. Now, an enzymatic strategy for ATP-dependent activation of protein and peptide C termini has been developed. This versatile tool can be deployed for synthesis of C-terminal thioesters that enhance the yield and accessibility of diverse protein bioconjugation methods.

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一种用于高产、atp驱动的c端硫酯生成的酶

目前用于蛋白质c端生物偶联的工具产量有限。现在，一种atp依赖的蛋白质和肽C末端激活的酶策略已经被开发出来。该多功能工具可用于合成c端硫酯，从而提高了多种蛋白质生物偶联方法的产率和可及性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature chemistry 化学-化学综合

CiteScore

29.60

自引率

1.40%

发文量

226

审稿时长

1.7 months

期刊介绍： Nature Chemistry is a monthly journal that publishes groundbreaking and significant research in all areas of chemistry. It covers traditional subjects such as analytical, inorganic, organic, and physical chemistry, as well as a wide range of other topics including catalysis, computational and theoretical chemistry, and environmental chemistry. The journal also features interdisciplinary research at the interface of chemistry with biology, materials science, nanotechnology, and physics. Manuscripts detailing such multidisciplinary work are encouraged, as long as the central theme pertains to chemistry. Aside from primary research, Nature Chemistry publishes review articles, news and views, research highlights from other journals, commentaries, book reviews, correspondence, and analysis of the broader chemical landscape. It also addresses crucial issues related to education, funding, policy, intellectual property, and the societal impact of chemistry. Nature Chemistry is dedicated to ensuring the highest standards of original research through a fair and rigorous review process. It offers authors maximum visibility for their papers, access to a broad readership, exceptional copy editing and production standards, rapid publication, and independence from academic societies and other vested interests. Overall, Nature Chemistry aims to be the authoritative voice of the global chemical community.