Low-density neutrophils: A critical component in understanding the tumor microenvironment of oral squamous cell carcinoma.

Q3 Medicine

Journal of Oral and Maxillofacial Pathology Pub Date : 2025-04-01 Epub Date: 2025-06-30 DOI:10.4103/jomfp.jomfp_351_24

Thayalan Dineshkumar, Nirmala Anandan, Rajkumar Krishnan, Indumathi Nandhakumar

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引用次数: 0

Abstract

Background: Tumour microenvironment is one of the evading research studies in the progression of cancers. Circulating neutrophil's contribution to tumor growth and metastatic progression has been a matter of research interest. The phenotypic subset of low-density neutrophils (LDNs) has been seen as closely associated with disease severity in various cancers.

Aim and objectives: This study aims to investigate the LDN and normal-density neutrophil (NDN) populations in oral cancer.

Materials and methods: This is prospective research in which the CD16+ population was magnetically sorted and leukocytes were separated in a density gradient. Using a flow cytometer, the percentages of the neutrophil subpopulation were ascertained. It was established that there are two distinct populations of human neutrophils: LDNs and NDNs.

Results: Patients with oral squamous cell carcinoma (OSCC) showed a higher percentage of LDN/NDN ratio and a correspondingly lower percentage of LDN/NDN ratio when compared to the control group. In addition, patients with OSCC had higher neutrophil-to-lymphocyte ratios (NLRs).

Conclusion: The current study showed a significant correlation between the LDN/NDN ratio in OSCC, which could be used as a benchmark for novel treatments. Additionally, the acquired LDNs/NDNs and NLR levels in cancer patients demonstrate their importance in OSCC patient prognosis and diagnosis.

查看原文本刊更多论文

低密度中性粒细胞：了解口腔鳞状细胞癌肿瘤微环境的关键组成部分。

背景：肿瘤微环境是肿瘤进展研究的热点之一。循环中性粒细胞对肿瘤生长和转移进展的贡献一直是研究兴趣的问题。低密度中性粒细胞（ldn）的表型亚群被认为与各种癌症的疾病严重程度密切相关。目的和目的：本研究旨在探讨口腔癌中LDN和正常密度中性粒细胞（NDN）群体的变化。材料和方法：这是一项前瞻性研究，CD16+群体被磁性分类，白细胞在密度梯度中分离。使用流式细胞仪，确定中性粒细胞亚群的百分比。已经确定存在两种不同的人类中性粒细胞群体：ldn和ndn。结果：口腔鳞状细胞癌（OSCC）患者的LDN/NDN比值比对照组高，相应的LDN/NDN比值比对照组低。此外，OSCC患者有较高的中性粒细胞与淋巴细胞比率（NLRs）。结论：本研究显示，OSCC的LDN/NDN比值具有显著相关性，可作为新治疗方法的基准。此外，肿瘤患者获得性ldn / ndn和NLR水平在OSCC患者预后和诊断中具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral and Maxillofacial Pathology Medicine-Otorhinolaryngology

CiteScore

1.40

自引率

0.00%

发文量

115

期刊介绍： The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.